2 × Syr Green QPCR Mix (nge-ROX ephezulu)

Incazelo emfushane:

Lo mkhiqizo, 2

XHUMANA NATHI

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Izici zomkhiqizo

Lo mkhiqizo, 2
I-SYBR eluhlaza engiyidaya idayi kaGreen Fluorescent obopha i-DNA ekhuphuke kabili (DOW-Strand DNA, i-DSDNA) I-Double Helix Grovels eFree State, kepha uma ibopha i-DNA ekhuphukile kabili, i-fluorescence yayo ithuthukiswa kakhulu. Lokhu kwenza ukuthi kunciphise inani le-DNA ekhutshwe kabili ekhiqizwa ngesikhathi se-PCR Amplification ngokuthola ubukhulu be-fluorescence.
I-ROX isetshenziswa njengodayi lokulungiswa ukuze kulungiswe ukuguquguquka kokuguquguquka kwe-fluorescence kungahlali ku-PCR, ngaleyo ndlela kunciphise umehluko wendawo. Umehluko onjalo ungabangelwa yizici ezahlukahlukene, njengephutha le-pipette noma ukuhwamuka kwesampula. Amathuluzi we-Fluorescence ahlukene anezidingo ezihlukile ze-ROX, futhi lo mkhiqizo ulungele ukuhlaziya okuhlosiwe kwe-fluorescence okudinga ukulungiswa okuphezulu kwe-ROX.

Izingxenye eziphambili

Num.	Ukuqoqa	Ukucacisa	Ubuningi	Izingxenye eziphambili
1	2 × Syr Green QPCR Mix (nge-ROX ephezulu)	I-1ml	5	I-Dntps, ama-Tris Buffers, i-ROX ephezulu, i-TAQ DNA polymerase, eSybr Green I Dyes, MG2 +