2× SYBR eluhlaza okotshani qPCR MIX (Nge-High ROX)

Izici zomkhiqizo

Lo mkhiqizo, 2×SYBR oluhlaza okotshani we-qPCR MIX, uza ngeshubhu elilodwa eliqukethe zonke izingxenye ezidingekayo zokukhulisa nokutholwa kwe-PCR, okuhlanganisa i-Taq DNA poLymerase, udayi we-SYBR oluhlaza we-SYBR, Idayi Yereferensi Ye-ROX Ephezulu, i-dNTPs, i-Mg2+, nebhafa ye-PCR.
Udayi we-SYBR oluhlaza okotshani we-fluorescent ohlanganisa i-DNA enemicu ekabili (i-double-strand DNA, dsDNA) isifunda se-double helix minor groove.SYBR eluhlaza I-fluoresces ibuthakathaka endaweni ekhululekile, kodwa uma ibophezela ku-DNA enemicu ekabili. , i-fluorescence yayo ithuthukiswa kakhulu. Lokhu kwenza kube nokwenzeka ukulinganisa inani le-DNA ephindwe kabili ekhiqizwa ngesikhathi sokukhulisa i-PCR ngokuthola amandla e-fluorescence.
I-ROX isetshenziswa njengodayi wokulungisa ukulungisa ukuguquguquka kwe-fluorescence okungahlobene ne-PCR, ngaleyo ndlela kuncishiswe umehluko wendawo. Umehluko onjalo ungabangelwa izici ezihlukahlukene, njengephutha le-pipette noma ukuhwamuka kwesampula. Amathuluzi ahlukene we-fluorescence quantification anezidingo ezihlukile ze-ROX, futhi lo mkhiqizo ulungele abahlaziyi be-fluorescence quantification abadinga ukulungiswa kwe-High ROX.