I-2 × SYBR green qPCR MIX （Ene-High ROX）

Izici zomkhiqizo

Lo mkhiqizo, i-2×SYBR green qPCR MIX, ufika ngethubhu elilodwa eliqukethe zonke izingxenye ezidingekayo ukuze kukhuliswe futhi kutholakale i-PCR, okuhlanganisa i-Taq DNA poLymerase, i-SYBR green I dye, i-High ROX Reference Dye, i-dNTPs, i-Mg2+, kanye ne-PCR buffer.
Idayi eluhlaza okwesibhakabhaka i-SYBR iyidayi eluhlaza okwesibhakabhaka ebopha kwi-DNA enezintambo ezimbili (i-DNA enezintambo ezimbili, i-dsDNA) double helix minor groove region. I-SYBR green I ikhanya kancane esimweni samahhala, kodwa uma ibopha kwi-DNA enezintambo ezimbili, ukukhanya kwayo kuyathuthuka kakhulu. Lokhu kwenza kube nokwenzeka ukulinganisa inani le-DNA enezintambo ezimbili ekhiqizwa ngesikhathi sokukhulisa i-PCR ngokuthola ukuqina kwe-fluorescence.
I-ROX isetshenziswa njengodayi wokulungisa ukulungisa ukuguquguquka kwe-fluorescence okungahlobene ne-PCR, ngaleyo ndlela kunciphisa umehluko wendawo. Umehluko onjalo ungabangelwa yizinto ezahlukahlukene, njengephutha le-pipette noma ukuhwamuka kwesampula. Izinsimbi ezahlukene zokulinganisa i-fluorescence zinezidingo ezahlukene ze-ROX, futhi lo mkhiqizo ufanelekela abahlaziyi be-fluorescence quantification abadinga ukulungiswa kwe-High ROX.