Ijeli ye-Agarose Entsha Yomkhiqizo we-Bigfish Iyatholakala Emakethe

Incazelo emfushane:

Amabhendi aphephile, asheshayo, amahle
Ijeli ye-agarose eqanjwe kusengaphambili ye-Bigfish isiyatholakala manje
Ijeli ye-agarose elungiselelwe kusengaphambili

XHUMANA NATHI

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Isingeniso Somkhiqizo

Ijeli ye-agarose elungiselelwe kusengaphambili uhlobo lwepuleti lejeli ye-agarose elilungiselelwe kusengaphambili, elingasetshenziswa ngqo ekuhlolweni kokuhlukanisa nokuhlanzwa kwama-macromolecule ezinto eziphilayo njenge-DNA. Uma kuqhathaniswa nendlela yendabuko yokulungiselela ijeli ye-agarose, ijeli ye-agarose elungiselelwe kusengaphambili inezinzuzo zokusebenza okulula, ukonga isikhathi kanye nokuzinza okuhle, okungathuthukisa kakhulu ukusebenza kahle kokuhlola, kunciphise ukuguquguquka kokuhlola, futhi kuvumele abacwaningi ukuthi bagxile kakhulu ekutholeni nasekuhlaziyeni imiphumela yokuhlolwa.

Imininingwane

Imikhiqizo yejeli ye-agarose eyenziwe kusengaphambili eyenziwe yiBigfish isebenzisa idayi ye-nucleic acid engeyona enobuthi ye-GelRed, efanelekile ukuhlukaniswa kwama-nucleic acid asukela ku-0.5 kuya ku-10kb ubude. Ijeli ayinayo i-DNase, i-RNase kanye ne-Protease, kanti amabhande e-nucleic acid ayizicaba, acacile, athambile futhi anesisombululo esiphezulu.