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Ikhithi yokuhlanza ye-Magpid Plasmid DNA
Isingeniso Esifushane
Le kit yamukela uhlelo oluthile lwe-buffer oluqalile futhi oluhlelekile nokwenziwa ngobuhlalu obunobunzima obubopha ngokuqondile ku-DNA, olungabopha ngokushesha, adsorb, luhlukanise futhi luhlanze ama-nucleic acid. Ilungele kakhulu ukwehlukanisa ngokushesha nangokufanele nokuhlanza i-Plasmid DNA kusuka ku-0.5-2ml (ejwayelekile 1-1.5ml) uketshezi lwamagciwane, ngenkathi isusa izinsalela ezinjengamaprotheni nama-ion eon. Ngokuxhasa ukusetshenziswa kweBigfish Magfish Bead Nucleic Acid Extractor, ilungele kakhulu ukukhishwa okuzenzakalelayo kosayizi abakhulu besampula. I-Plasmid ye-Plasmid ekhishwe inobumsulwa obuphezulu nekhwalithi enhle, futhi ingasetshenziswa kabanzi ekuhlolweni okuphansi okufana nokugaya kwe-enzyme, i-inzyme, ukuguqulwa, ama-nds, njll..
Izici zomkhiqizo
Ikhwalithi enhle:Hlanganisa futhi uhlanze i-Plasmid DNA kusuka kukhambi le-0.5-2ml bacterial ngesivuno esiphezulu nobumsulwa obuhle.
Ngokushesha futhi kulula:Yonke inqubo ayidingi i-centrifugation ephindaphindwayo noma imisebenzi ye-filltration, okwenza kube lula ukukhishwa osayizi abakhulu besampula.
Kuphephile futhi okungelona ubuthi:Akunasidingo se-anobuthi anobuthi ama-reagents anobuthi afana ne-phenol / chloroform.
-JwayelisekaIzinsimbi
IBigfish: BFEX-32E, BFEX-32, BFEX-96E, Bfex-16e
Ukucaciswa komkhiqizo
| UmphumelaNangulo | Ikati. Cha. | Ukufaka ndawonye |
| I-Magpire Plasmid DNA yokuhlanza Ikhithi yokuhlanza (iphakheji egcwaliswe ngaphambilini) | BFMP09R | I-32t |
| I-Magpire Plasmid DNA yokuhlanza Ikhithi yokuhlanza (iphakheji egcwaliswe ngaphambilini) | BFMP09R1 | 40komisakavu |
| I-Magpire Plasmid DNA yokuhlanza Ikhithi yokuhlanza (iphakheji egcwaliswe ngaphambilini) | BFMP09R96 | 9KOKWE |
| I-RNASEA(thengaIsihlehlukene | I-Bfrd017 | I-1ml / Tube(I-10mg / mlIsihlehlukene |
Izilungiselelo Zobumfihlo