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Ilayisi lingenye yezitshalo ezibaluleke kakhulu, ezitholakala ezitshalweni zasemanzini ezikhula emanzini zomndeni wakwaPoaceae. IShayina ingenye yezindawo zokuqala zokuhlala zelayisi, ezitshalwa kabanzi eningizimu yeShayina kanye nesifunda saseNyakatho-mpumalanga. Ngokuthuthuka kwesayensi nobuchwepheshe, amasu esimanje ebhayoloji yama-molecular asetshenziswa kabanzi ocwaningweni ngelayisi. Ukuthola i-DNA ye-genomic yelayisi esezingeni eliphezulu, ehlanzekile kakhulu kubeka isisekelo esiqinile sezifundo zofuzo ezingezansi. I-BigFish Sequence Magnetic Bead-Based Rice Genomic DNA Purification Kit ivumela abacwaningi belayisi ukukhipha i-DNA yelayisi kalula, ngokushesha, nangokusebenza kahle.
Ikhithi Yokuhlanza I-DNA Ye-Rice Genome
Ukubuka konke komkhiqizo:
Lo mkhiqizo usebenzisa uhlelo lwe-buffer oluyingqayizivele oluthuthukiswe ngokukhethekile nolulungiselelwe kahle kanye nama-magnetic beads anezakhiwo ezithile zokubopha i-DNA. Ubopha ngokushesha, umunce, futhi uhlukanise ama-nucleic acid ngenkathi ususa ngempumelelo ukungcola okufana nama-polysaccharides kanye nama-polyphenolic compounds ezitshalweni. Ufaneleka kakhulu ekukhipheni i-genomic DNA ezicutshini zamaqabunga ezitshalo. Umataniswe ne-BigFish Magnetic Bead Nucleic Acid Extraction Instrument, ulungele ukukhipha ngokuzenzakalelayo amavolumu amakhulu esampula. Imikhiqizo ye-nucleic acid ekhishwe ibonisa ubumsulwa obuphezulu kanye nekhwalithi enhle kakhulu, okwenza ukuthi isetshenziswe kabanzi ocwaningweni lokuhlola olungezansi njenge-PCR/qPCR kanye ne-NGS.
Izici Zomkhiqizo:
Kuphephile futhi akunabo ubuthi: Asikho isidingo sama-reagent anobuthi e-organic njenge-phenol/chloroform
I-high-throughput ezenzakalelayo: Ihlanganiswe ne-Beagle sequencing nucleic acid extractor, ingenza ukukhipha okuphezulu futhi ifanele ukukhipha osayizi abakhulu besampula
Ubumsulwa obuphezulu kanye nekhwalithi enhle: Umkhiqizo okhishwe uhlanzekile kakhulu futhi ungasetshenziswa kuma-NGS angezansi, ukuhlanganiswa kwama-chip kanye nezinye izivivinyo.
Izinsimbi ezihambisanayo: I-BigFish BFEX-32/BFEX-32E/BFEX-96E
Isikhathi sokuthunyelwe: Septhemba 11-2025
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