中文网站
Emasimini e-ukuxilongwa kwezokwelapha ngaphakathi kwesibeletho (IVD), ukwakheka kwezakhi zofuzo, kanye nocwaningo lwama-molecule, amasampula omlomo—njengeama-swab omlomo, ama-swab omphimbo, namathe— zisetshenziswa kabanzi ekuhlolweni kwe-nucleic acid ngenxa yezizathu zazo.ukuqoqwa okulula, imvelo engahlaseli, kanye nenqubo yokuthatha amasampula engenabuhlunguNoma kunjalo, amasampula omlomo ngokuvamile aquketheinani elilinganiselwe lama-nucleic acidfuthi bavame ukungcoliswa yi-amaprotheni nokunye ukungcolaIzindlela zokukhipha zendabuko zivame ukuhlushwa yi-imisebenzi eyinkimbinkimbi, ukusebenza kahle okuphansi, kanye nokusetshenziswa kwama-reagents anobuthi, okungalimaza kakhulu ukunemba nokuzinza kwezinhlelo zokusebenza ezingezansi ezifanaI-PCR/qPCR kanye nokulandelana kwesizukulwane esilandelayo (NGS).
IIkhithi Yokukhipha I-DNA Yomlomo Esekelwe Kuma-Magnetic Bead ye-BFMP06, yathuthukiswa yi-I-Hangzhou Bigfish FeiXu Biotechnology, inikezaikhambi eliphephile, elisebenzayo, nelithembekileyokukhipha i-DNA yesampula yomlomo. Ngomklamo wayo wobuchwepheshe obusha kanye nezindinganiso zokusebenza eziqinile, le khithi isibe ithuluzi elithembekile kokubili kuma-laboratory ezokwelapha nawocwaningo.
Ikhithi ye-BFMP06 yakhelwe eduze kwe-uhlelo lwe-buffer oluhlelwe ngendlela ehlukilekuhlanganisweUbuhlalu be-hydroxyl magnetic obuqondene ne-DNA, kwakha umsebenzi wokuhlanza i-nucleic acid osebenza kahle kakhulu. Ngemva kokuba isampula ihlungiwe ku-lysis buffer, izingxenye zamaseli ziyaphazamiseka bese kukhishwa ama-nucleic acid. Amaqembu asebenzayo ebusweni be-magnetic beads akhetha ukubopha i-DNA yamahhala, akha i-stableama-complex e-magnetic bead–DNA.
Ngaphansi kwensimu yamagnetic yangaphandle, lezi zakhiwo ziyadlulaizinyathelo ezimbili eziqondile zokugezaukususa ngokuphelele amaprotheni, usawoti, kanye nezinye izinto ezingcolisayo. Okokugcina,i-DNA ye-genomic emsulwa kakhuluisuswa kahle kusetshenziswa i-elution buffer.
Incazelo Yomkhiqizo
Lo mkhiqizo usebenzisa uhlelo lwe-buffer oluthuthukiswe ngokukhethekile futhi olulungiselelwe kahle kanyeubuhlalu obunamandla obuhlanganisa i-DNA ngqo, okuvumela ukumuncwa okusheshayo, ukuhlukaniswa, kanye nokuhlanzwa kwama-nucleic acid. Ifaneleka kakhuluukuhlukaniswa okusheshayo nokuphumelelayo kwe-DNA ye-genomic kusuka kuma-swabs omlomo, ama-swabs omphimbo, kanye namasampula amathe, kuyilapho kususwa ngempumelelo amaprotheni asele kanye nosawoti.
Uma isetshenziswa kanyekanyeIzinsimbi zokukhipha i-nucleic acid ezisuselwe ku-Bigfish FeiXu magnetic bead, ikhithi ilungele kakhuluukukhipha okuzenzakalelayo okuphezuluI-DNA ye-genomic ehlanziwe iwuhlobo lwe-ubumsulwa obuphezulu kanye nekhwalithi enhle kakhulu, okwenza ifaneleke ezinhlotsheni eziningi zezinhlelo zokusebenza ezingezansi, okuhlanganisaI-PCR/qPCR kanye ne-NGS.
Izici Zomkhiqizo
Ikhwalithi ephezulu
Ihlukanisa futhi ihlanze i-DNA ye-genomic ngempumeleloama-swab omlomo, ama-swab omphimbo, namathe, ukulethaisivuno esikhulu kanye nobumsulwa obukhulu.
Okusheshayo Nokulula
Akudingeki izinyathelo zokuhlunga nge-centrifugation noma i-vacuum eziphindaphindwayo. Iyahambisana namathuluzi okukhipha azenzakalelayo, okwenza kube kuhle kakhuluukucutshungulwa kwesampula okukhulu.
Kuphephile Futhi Akunabo Ubuthi
Asikho isidingo sama-reagent anobuthi e-organic afanai-phenol noma i-chloroform.
Izinsimbi Ezihambisanayo
I-Bigfish FeiXu BFEX-16E
I-BFEX-32
I-BFEX-32E
I-BFEX-96
Imiphumela Yokuhlola
Amasampula e-swab yomlomo (afakwe emanziniIsixazululo sokulondolozwa esingu-400 μL) kanye namasampula amathe (Isixazululo sokugcina esingu-200 μL samathe + 200 μL) zicutshungulwe kusetshenziswaI-Bigfish FeiXu Oral Swab Genomic DNA Purification Kit. I-DNA yaphunyuka ngoI-buffer yokulungisa engu-70 μLfuthi yahlaziywa yi-i-electrophoresis yejeli ye-agarose, njengoba kuboniswe esithombeni esingezansi.
M: Uphawu lwe-DNA (2K Plus II)
Imininingwane Yomkhiqizo
Isikhathi sokuthunyelwe: Jan-15-2026
Izilungiselelo zobumfihlo