Ukuhlolwa kwe-DNA methylation kuhlangene nama-Smartphones okuhlola kuqala kwamathumbu kanye nokuhlolwa kwe-leukemia ngokunemba kwe-90.0%!

Ukutholwa kusenesikhathi komdlavuza kususelwa ku-biopsy ye-liquid kuyindlela entsha yokutholwa komdlavuza nokuxilongwa okuhlongozwe yi-US Naniac Cancer Institute yase-US eminyakeni yamuva, ngenhloso yokuthola umdlavuza wokugcina noma ngisho nezilonda ezingezinhle. Kusetshenziswe kabanzi njenge-biomarker yenoveli yokuxilongwa kusenesikhathi kwama-milignignancies ahlukahlukene, kufaka phakathi umdlavuza wamaphaphu, ama-tumortuintenal Tumors, ama-gliomas kanye nama-gynecological tumors.

Ukuvela kwamapulatifomu ukukhomba i-methylation landscape (methylscape) i-biomarkers inamandla okuthuthukisa kakhulu ukuhlolwa okukhona okukhona komdlavuza, ukubeka iziguli esigabeni sokuqala esiyelapheka.

Muva nje, abacwaningi bathuthukise ipulatifomu elula futhi eqondile yokuzwakala kwe-methylation landscape ukutholwa okusekelwe kuma-nanoparticles we-cysteamine (cyst / aunps) ahlanganiswe ne-biosensor esekwe kwi-smartphone eyenza ukuhlolwa okususelwa kububanzi bezinhlobonhlobo zama-tumors ahlukahlukene. Ukuhlolwa kusenesikhathi kwe-leukemia kungenziwa kungakapheli imizuzu eyi-15 ngemuva kokukhishwa kwe-DNA kusampula yegazi, ngokunemba kuka-90.0%. Isihloko se-athikili ukutholwa okusheshayo kwe-Cancer DNA egazini lomuntu usebenzisa i-AUSPS ye-Cysteamine kanye ne-smartphone yokufunda ngomshini.

Umdwebo 1. Ipulatifomu elula futhi esheshayo yokuzwakala komdlavuza nge-Cyst / AUSPS Izakhi zenziwa ngezinyathelo ezimbili ezilula.

Lokhu kukhonjiswa kuMfanekiso 1. Okokuqala, kusetshenziswe isisombululo esinamanzi ukuqeda izingcezwana ze-DNA. I-Cyst / AUSPS yangezwa kwikhambi elixubile. I-DNA ejwayelekile ne-Malignant inezakhiwo ezihlukile ze-methylation, okuholela ezingcupheni ze-DNA ngezindlela ezahlukahlukene zokuzijabulisa. Ama-DNA ajwayelekile ahlanganisa ngokukhululekile futhi ekugcineni ahlanganise i-cyst / aunc, ephumela emvelweni eguqulwe kabusha ye-cyst / auuns, ukuze ushintsho ngombala kusuka kokubomvu kuya kuso iso ngeso le-Purple. Ngokuphambene nalokho, iphrofayili eyingqayizivele ye-methylation ye-Cancer DNA iholela ekukhiqizweni kwamaqoqo amakhulu ezinhlamvu ze-DNA.

Izithombe ezingama-96-kahle zithathwe kusetshenziswa ikhamera ye-smartphone. UCancer DNA ulinganiswe yi-smartphone efakwe ngokufunda komshini ngokuqhathaniswa nezindlela ezenzelwe ama-spectroscopy.

Ukuhlolelwa umdlavuza kumasampula egazi wangempela

Ukwelula ukusetshenziswa kwesiteji sokuzwakala, abaphenyi basebenzisa inzwa ehlukanise ngempumelelo phakathi kwe-DNA ejwayelekile neyamdlavuza kumasampula egazi angempela. Amaphethini we-methylation kumasayithi e-CPG alawula ukuvezwa kofuzo. Cishe kuzo zonke izinhlobo zomdlavuza, izinguquko ku-DNA methylation futhi ngenxa yalokho ekuboniseni izinhlobo zofuzo ezikhuthaza iTumourigenesis ukuthi zibonwe ukushintshana.

Njengemodeli yeminye imidlavuza ehambisana ne-DNA Methylation, abacwaningi basebenzise amasampula egazi ezigulini ze-leukemia kanye nokulawulwa okunempilo ukuphenya ukusebenza kwe-methamic sendscape ekuhlukaniseni umdlavuza we-leumiction. Le methylation yemvelo ye-biomarker ayigcini nje ngokuphuma kwezindlela ezisheshayo ezikhona ze-leukemia ezisheshayo, kodwa futhi ikhombisa ukuthi kungenzeka ukuthi kunwetshwe ekutholeni kusenesikhathi kwenqwaba yemidlakhadla ehlukahlukene kusetshenziswa lokhu okulula nokuqondile.

I-DNA kusuka kumasampula egazi kusuka ezigulini ezingama-31 zelekamia kanye nabantu abangu-12 abanempilo bahlaziywa. Njengoba kukhonjisiwe ebhokisini uPlot kuMfanekiso 2A, ukumunca okuhlobene kwamasampula omdlavuza (ΔA650 / 525) kwakuphansi kunaleyo ye-DNA kumasampula ajwayelekile. Lokhu kungenxa ye-hydrophopobicicity ethuthukisiwe eholela ekuhlanganiseni okunobunye be-Cancer DNA, okuvimbele ukuhlanganiswa kwama-cyst / aunc. Ngenxa yalokhu, lezi zingqimba zahlakazwa ngokuphelele engxenyeni engaphandle yomdlavuza ohlanganise, okwaphumela ekuhlakazekeni okuhlukile kwama-cyst / aunps adgresed on adserance ajwayelekile kanye nomdlavuza ama-DNA agcumbe. I-ROC Curves yabe seyikhiqizwa ngokuhlukahluka kombundu kusuka kunani elincane le-ΔAA650 / 525 kuya kunani eliphakeme.

Umdwebo 2. (a) Amanani Angaphansi Amunctional Amuncsance wezixazululo zeCyst / AunPS ekhombisa ubukhona be-Norrance (Blue) kanye nomdlavuza (obomvu) we-DNA ngaphansi kwezimo ezenziwe kahle

(Da650 / 525) wezindawo zebhokisi; (b) Ukuhlaziywa kwe-ROC kanye nokuhlola izivivinyo zokuxilonga. (c) I-Matrix edidekayo yokuthola iziguli ezijwayelekile nomdlavuza. .

Njengoba kukhonjisiwe kuMdwebo 2B, indawo ngaphansi kwe-Roc Curve (AUC = 0.9274) etholakele inzwa ethuthukisiwe ikhombisa ukuzwela okuphezulu nokucaciswa. Njengoba kungabonakala kusukela ebhokisini lebhokisi, iphuzu eliphansi kakhulu elimele iqembu le-DNA elijwayelekile alihlukaniswe kahle nephuzu eliphakeme kakhulu elimele iqembu le-Cancer DNA; Ngakho-ke, ukuguquguquka okunengqondo kwakusetshenziselwa ukuhlukanisa phakathi kwamaqembu ajwayelekile nomdlavuza. Njengoba kunikezwe isethi yokuguquguquka okuzimele, kulinganisa amathuba okuba umcimbi owenzeka, njengomdlavuza noma iqembu elijwayelekile. Amabanga ahlukahlukene athembekile phakathi kuka-0 no-1. Umphumela walokho kungenzeka ukuthi kungenzeka. Sinqume amathuba okukhonjwa komdlavuza (ikhasi) ngokususelwa ku-ΔAA650 / 525 ngokulandelayo.

lapho b = 5.3533, w1 = -6.965. Ukuhlukaniswa kwesampula, amathuba angaphansi kuka-0.5 akhombisa isampula evamile, ngenkathi amathuba angu-0.5 noma ngaphezulu akhombisa isampula yomdlavuza. Umdwebo 2c uveze i-matrix yokudideka eyenziwe kusuka ekushisweni kwekhefu-i-sodwa, eyayisetshenziselwa ukuqinisekisa ukuqina kwendlela yokuhlukanisa. Umdwebo 2D ufingqa ukuhlolwa kokuhlolwa kokuhlolwa kwendlela, kufaka phakathi ukuzwela, ukucaciswa, inani elifanele lokubikezela (i-PPV) nenani elingezinhle lokubikezela (NPV).

Ama-biosensors asuselwa kwi-smartphone

Ukuqhubeka nokwenza lula ukuhlolwa kwesampula ngaphandle kokusebenzisa ama-spectrophotometer, abacwaningi basebenzise ubuhlakani bokufakelwa (AI) ukuhumusha umbala wekhambi futhi bahlukanise phakathi kwabantu abajwayelekile nabanemdlavuza. Njengoba kunikezwe lo, umbono wekhompyutha wasetshenziselwa ukuhumusha umbala wekhambi le-cyst / aunps kwi-DNA ejwayelekile (onsomi) noma umdlavuza (obomvu) usebenzisa izithombe zamapuleti angu-96 athathwe ngekhamera yefoni ephathekayo. I-Artificial Intelligence inganciphisa izindleko futhi ithuthukise ukufinyeleleka ekuhumusheni umbala we-nanoparticle solution, futhi ngaphandle kokusebenzisa izesekeli ze-smartphone ze-optical hardware. Ekugcineni, amamodeli wokufunda umshini amabili, kufaka phakathi ihlathi elingahleliwe (i-RF) nomshini we-vector wokusekela (i-SVM) aqeqeshelwe ukwakha amamodeli. Zombili amamodeli we-RF ne-SVM ahlukanise kahle amasampula anempilo futhi engemihle ngokunemba kwama-90.0%. Lokhu kuphakamisa ukuthi ukusetshenziswa kobuhlakani bokufakelwa kuma-biosens asuselwa kumakhalekhukhwini kungenzeka.

Umdwebo 3. (a) Isigaba esiqondiwe sesixazululo esirekhodiwe ngesikhathi sokulungiswa kwesampula yesinyathelo sokutholwa kwesithombe. (b) Isibonelo esithathwe ngesithombe sokutholwa kwesithombe. .

Usebenzisa i-Cyst / AUSPS, abacwaningi bathuthukise ngempumelelo isiteji sokuzwa esilula se-methylation landscape detection kanye nenzwa ekwazi ukuhlukanisa i-DNA ejwayelekile kusuka ku-Cancer DNA lapho besebenzisa amasampula egazi angempela wokuhlola i-leukemia. Inzwa ethuthukisiwe ikhombisile ukuthi i-DNA ekhishwe amasampula egazi langempela ikwazile ukuthola ngokushesha futhi ikwazi ukuthola kahle inani elincane le-Cancer DNA (3nm) ezigulini ze-leukemia ngemizuzu eyi-15, futhi ikhombise ukunemba kuka-95.3%. Ukuze uqhubeke nokwenza lula ukuhlolwa kwesampula ngokuqeda isidingo se-spectrophotometer, ukufundwa komshini kusetshenziselwa ukuhumusha umbala wekhambi futhi uhlukanise phakathi kwabantu ojwayelekile nangomdlavuza usebenzisa i-90.0%.

Inkomba: Doi: 10.1039 / D2RA05725E