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Inqubekelaphambili ehlobene
Ngokusho kweHhovisi Lolwazi loMnyango Wezolimo nezindawo zasemakhaya, ngo-Agasti 2018, isifo sezingulube sase-Afrika senzeke e-Shenbei New District, eDolobheni laseShenyang, eSifundazweni sase-Liaoning, okuwubhubhane lwezingulube lokuqala lwase-Afrika eChina. Kusukela ngoJanuwari 14, 2019, isifo sezingulube sase-Afrika senzeke ezifundazweni ezingaphezu kuka-20 eChina, sabulala izingulube ezingu-916000, okubangele ukukhathazeka komphakathi.
I-African swine fever (ASF)
I-ASF(i-African Swine fever) isifo esithathelwanayo esiyingozi esibangelwa i-African swine fever virus (ACFV) ehlasela izingulube ezifuywayo nezingulube zasendle (izingulube zasendle zase-Afrika, izingulube zasendle zaseYurophu, njll.). Inhlangano yomhlaba yezempilo yezilwane (OIE) isibalule njengesifo sezilwane esibikwe ngokomthetho, okuwuhlobo lobhubhane lwezilwane iChina egxile ekulugwemeni.
Izici zesifo
Ukubonakaliswa kwemitholampilo imfiva (kufika ku-40 ~ 42 ℃), i-tachycardia, i-dyspnea, ukukhwehlela okuncane, ukuphuma kwe-serous noma i-mucinous purulent emehlweni nasemakhaleni, i-cyanosis yesikhumba, ukopha okusobala kwezinso, i-lymph node kanye ne-mucosa yamathumbu. Izimpawu zomtholampilo ze-African classical swine fever ziyefana nalezo ze-classical swine fever, ezingatholwa kuphela ngokuqapha elabhorethri.
Isixazululo se-African classical swine fever
1. Ukucubungula isampula
Ifanele igazi kanye nezicubu ezihlukahlukene zezingulube okusolakala ukuthi ziyagula: i-spleen, i-lymph node kanye nezicubu zezinso.
Amasampula egazi
Thatha isampula legazi elingu-200 μ L, 5000g centrifugal 5 min, thatha i-supernatant iyohlolwa.
Amasampula ezicubu
Ngemva kokuba amasampula ezicubu esegaywe ngokugcwele, inani elifanele likasawoti ovamile noma i-PBS lanezelwa, futhi i-supernatant yafakwa i-centrifuged ukuze ihlolwe.
2. Ukukhipha okuzenzakalelayo
I-Hangzhou Bigfish Bio-tech Co., Ltd. I-BFEX-32 i-automatic nucleic acid extractor ingaqedela ukukhishwa kwamasampula angu-32 emizuzwini engu-30, engahlangabezana nezimfuneko zakho zokukhipha inani elikhulu lamasampuli. Yonke le nqubo ayidingi noma yimuphi omunye umsebenzi, yonga isikhathi futhi inciphisa iphutha lokusebenza ngesandla, futhi ithuthukisa ukusebenza kahle komsebenzi ngangokunokwenzeka.
3. Ukuhlanzeka okuphezulu kwe-nucleic acid
Ikhithi yokukhipha i-MagPure Nucleic acid, ene-BFEX-32, umkhiqizo ufane ngokuphelele nokutholwa kwe-PCR ne-QPCR.
4. Ukuhlolwa kwekhompyutha nokuhlaziya
Ngokwesimo sangempela selabhorethri, umsebenzisi usebenzisa uhlelo lokuthola ikhwalithi noma inani.
I-Hangzhou Bigfish Bio-tech Co., Ltd. ihlinzeka ngekhithi yokuthola ikhwalithi (Yobuningi) ye-African classical swine fever (ACFV) ene-FC-96G (BFQP-16/48), ekwazi ukubona i-ACFV kabanzi, ngokuzwela nangokuthembekile.
Okuqukethwe okwengeziwe, sicela unake i-akhawunti esemthethweni ye-WeChat ye-Hangzhou Bigfish Bio-tech Co., Ltd.
Isikhathi sokuthumela: May-23-2021
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