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Inqubekela phambili ehlobene
Ngokusho kweHhovisi Lolwazi loMnyango Wezolimo nezindawo zasemakhaya, ngo-Agasti 2018, kwavela isifo sezingulube sase-Afrika eShenbei New District, eShenyang City, eSifundazweni saseLiaoning, okuyisifo sezingulube sokuqala sase-Afrika eShayina. Kusukela ngoJanuwari 14, 2019, isifo sezingulube sase-Afrika senzeke ezifundazweni ezingaphezu kuka-20 eShayina, sabulala izingulube ezingu-916000, okwabangela ukukhathazeka komphakathi.
Umkhuhlane wezingulube wase-Afrika (ASF)
I-ASF (i-African Swine fever) yisifo esithathelwanayo esibangelwa yigciwane le-African swine fever (ACFV) elithelela izingulube zasekhaya kanye nezingulube zasendle (i-African wild boar, i-European wild boar, njll.). Inhlangano yomhlaba wonke yezempilo yezilwane (i-OIE) iyibhale njengesifo sezilwane esibikiwe ngokomthetho, okuwuhlobo lobhubhane lwezilwane olugxile ekuvimbeleni iChina.
Izici zesifo
Izimpawu zomtholampilo umkhuhlane (kufika ku-40 ~ 42 ℃), i-tachycardia, ukuphelelwa umoya, ukukhwehlela okuyingxenye, ukukhishwa kwe-serous noma i-mucinous purulent emehlweni nasekhaleni, i-skin cyanosis, ukuphuma kwegazi okusobala kwezinso, i-lymph node kanye ne-mucosa yamathumbu. Izimpawu zomtholampilo zomkhuhlane wezingulube wase-Afrika zifana nezemfiva yezingulube yasendulo, engatholakala kuphela ngokuqapha kwelabhorethri.
Isixazululo somkhuhlane wezingulube wasendulo wase-Afrika
1. Ukucutshungulwa kwesampula
Ifanele igazi nezicubu ezahlukahlukene zezingulube ezisolwa ukuthi zigula: udende, i-lymph node kanye nezicubu zezinso.
Amasampula egazi
Thatha isampula yegazi engu-200 μ L, i-centrifugal engu-5000g imizuzu emi-5, thatha i-supernatant ukuze uyihlole.
Amasampula ezicubu
Ngemva kokuba amasampula ezicubu esegayiwe ngokuphelele, kwanezelwa inani elifanele le-saline evamile noma i-PBS, futhi i-supernatant yafakwa i-centrifuge ukuze ihlolwe.
2. Ukukhipha okuzenzakalelayo
I-Hangzhou Bigfish Bio-tech Co., Ltd. I-BFEX-32 automatic nucleic acid extractor ingaqedela ukukhipha amasampula angu-32 ngemizuzu engu-30, okungahlangabezana nezidingo zakho zokukhipha inani elikhulu lamasampula. Yonke inqubo ayidingi olunye uhlobo lomsebenzi, isindisa isikhathi futhi inciphisa iphutha lokusebenza ngesandla, futhi ithuthukisa ukusebenza kahle komsebenzi ngangokunokwenzeka.
3. Ukuhlanzwa kwe-nucleic acid okumsulwa okuphezulu
Ikhithi yokukhipha i-MagPure Nucleic acid, ene-BFEX-32, umkhiqizo ufana ngokuphelele nokutholwa kwe-PCR kanye ne-QPCR.
4. Ukuhlolwa nokuhlaziywa kwekhompyutha
Ngokwesimo sangempela selabhorethri, umsebenzisi usebenzisa uhlelo lokuthola ulwazi olunembile noma olunobuningi.
I-Hangzhou Bigfish Bio-tech Co., Ltd. ihlinzeka ngekhithi yokuthola i-qualitative (Quantitative) ye-African classical swine fever (ACFV) ene-FC-96G (BFQP-16/48), engathola i-ACFV kabanzi, ngozwelo nangokuthembekile.
Okuqukethwe okwengeziwe, sicela unake i-akhawunti esemthethweni ye-WeChat ye-Hangzhou Bigfish Bio-tech Co., Ltd.
Isikhathi sokuthunyelwe: Meyi-23-2021
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