I-Hangzhou Bigfish Bio-tech Co., Ltd. ithuthukise ngempumelelo ikhithi entsha yokuhlola iCoronavirus

01 Inqubekelaphambili yakamuva yesimo sobhubhane
NgoZibandlela wezi-2019, kwenzeka uchungechunge lwamacala e-viral pneumonia angachazwanga eWuhan. Lesi sigameko sasikhathazwe kakhulu yizo zonke izigaba zokuphila. I-pathogen ekuqaleni yahlonzwa njengegciwane leCorona Elisha futhi yaqanjwa ngokuthi “2019 New Corona virus (2019-nCoV)” yi-WHO.

I-WHO esitatimendeni sangomhla ziyi-16 ithe iwutholile umbiko ngecala legciwane leCorona eliqinisekiswe yiJapan. Lesi yisigameko sesibili ngemuva kokuba iThailand ithole icala le-New Corona virus, etholwe ngaphandle kwaseChina.

IKomidi Lezempilo likaMasipala waseWuhan likhiphe isekhula mhlaka-19 Novemba, lithi njengoba kubalwa kuze kube yihora le-24 mhlaka 17, uWuhan ubike izehlakalo ezingama-62 zenyumoniya edalwe yiNew Corona virus, kanti abangu-19 sebelashiwe base bededelwa, abayisishiyagalombili sebeshonile. belashelwa amacala anzima, amacala ama-2 ashonile, kanti ezinye iziguli zisesimweni esigculisayo. Iziguli zithola ukwelashwa zodwa ezibhedlela eziqokiwe eWuhan.

02 Liyini igciwane le-corona
I-Corona virus wuhlobo lwamagciwane oludala ikakhulukazi izifo zokuphefumula kanye nezifo zamathumbu. Lolu hlobo lwezinhlayiya zegciwane lunezinhlayiya eziningi ezihlelwe njalo ngaphezulu, futhi zonke izinhlayiya zegciwane zifana nomqhele wombusi, ngakho-ke kuthiwa "igciwane le-corona".

I-Severe acute Respiratory Syndrome (SARS-CoV) kanye ne-Middle East Respiratory Syndrome coronavirus (mers-cov), eziye zadala izifo eziwumshayabhuqe ezimbi phambilini, zingadala izifo ezimbi kakhulu zokuphefumula.

Isihlahla esisha se-phylogenetic seCoronavirus 2019-nCoV

03 Uhlelo lokuthola igciwane leCorona
I-Hangzhou Bigfish Bio-tech Co., Ltd. ibilokhu ilandela eduze inqubekelaphambili yalolu bhubhane kusukela kwaqubuka lesi sifo. Ngemuva kokumenyezelwa kokulandelana kofuzo lwegciwane le-Wuhan New Corona virus (2019-nCoV) yiziphathimandla zikahulumeni, ikhithi yokuthola i-New Corona virus 2019-nCoV ye-nucleic acid yathuthukiswa ngempumelelo okokuqala ngqa, ihlinzeka ngohlelo oluphelele lokuhlonza igciwane leCorona Elisha. ukutholwa.

Ukutholwa kwethagethi ekabili
Ngegciwane leCorona Elisha, kwasetshenziswa ama-probe primers ukuze kutholwe izingxenye ezimbili zesifunda ezithile, eziqinisekisa ukunemba kokutholakala futhi zivikele ngempumelelo ukutholakala okungatholakali.

Ukuzwela okuphezulu
I-probe primer ephindwe kabili ehlanganiswe ne-fluorescent probe entsha ingathuthukisa ngempumelelo ukuzwela kokutholwa kwekhithi, efaneleka kakhulu ukutholwa nokuxilongwa kweziguli zangaphambi kwesikhathi.

Ukutholwa okuzenzakalelayo
Ukusuka ekukhishweni kuye ekutholweni kokukhulisa, yonke isethi yama-reagents yasetshenziswa ukuthola ukutholwa okuzenzakalelayo.

Okuqukethwe okwengeziwe, sicela unake i-akhawunti esemthethweni ye-WeChat ye-Hangzhou Bigfish Bio-tech Co., Ltd.