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Amagciwane (amagciwane e-Biological) ayizinto eziphilayo ezingezona amaselula ezibonakala ngosayizi omncane, isakhiwo esilula, kanye nokuba khona kohlobo olulodwa kuphela lwe-nucleic acid (DNA noma i-RNA). Kumelwe abulale amangqamuzana aphilayo ukuze aziphindaphinde futhi ande. Uma ehlukaniswa namaseli awo abambayo, amagciwane aba izinto zamakhemikhali ezingenawo umsebenzi wokuphila futhi angakwazi ukuziphindaphinda okuzimele. Amakhono abo okuphindaphinda, ukulotshwa, kanye nokuhumusha konke kwenziwa ngaphakathi kweseli elisingethe.Ngakho, amagciwane akha isigaba sebhayoloji esiyingqayizivele esinazo zombili izici zamangqamuzana amakhemikhali kanye nezici zebhayoloji eziyisisekelo; zikhona njengezinhlayiya ezithathelwanayo ezingaphandle kwamaseli kanye nezakhi zofuzo eziphindaphindayo.
Amagciwane angawodwana mancane kakhulu, iningi lawo litholakala kuphela ngesibonakhulu se-electron. Amakhulu kunawo wonke, ama-poxviruses, alinganisa cishe ama-nanometres angu-300, kuyilapho amancane kunawo wonke, ama-circoviruses, angaba ngama-nanometer angu-17 ngosayizi. Kuyaziwa kabanzi ukuthi amagciwane amaningi abeka engcupheni enkulu impilo nokuphila kwabantu, njengenoveli i-coronavirus, igciwane lesifo sokusha kwesibindi kohlobo B (HBV), kanye negciwane le-human immunodeficiency virus (HIV). Nokho, amagciwane athile ebhayoloji nawo anikeza izinzuzo ezithile kubantu. Isibonelo, ama-bacteriophages angasetshenziswa ukwelapha izifo ezithile zamagciwane, ikakhulukazi lapho kubhekwana nama-superbugs lapho ama-antibiotic amaningi engasasebenzi.
Ngokuphazima kweso, sekuphele iminyaka emithathu kwaqala ubhubhane lwe-COVID-19. Kodwa-ke, ukuhlolwa kwe-nucleic acid kudlulela ngalé kokuthola i-coronavirus yenoveli. Ngale kwe-COVID-19, ukuhlolwa kwe-nucleic acid kusebenza njengezinga legolide lokutholwa ngokushesha nangokunembayo kwamagciwane amaningi, kuvikela impilo yethu ngokuqhubekayo. Ngaphambi kokuhlolwa kwe-nucleic acid, ukuthola ikhwalithi ephezulu, i-viral nucleic acid ehlanzekile kakhulu ibalulekile ukuze kuqinisekiswe ukunemba kwezinqubo zokuxilonga ezilandelayo.
Isingeniso Somkhiqizo
Ukubuka konke komkhiqizo:
Le khithi ihlanganisa ubuhlalu be-superparamagnetic kanye namabhafa wokukhipha akhiwe ngaphambilini, anikeza lula, ukucutshungulwa okusheshayo, isivuno esikhulu, nokukhiqiza kabusha okuhle kakhulu. I-DNA/RNA ye-viral genomic ewumphumela ayinayo amaprotheni, i-nuclease, noma okunye ukuphazamiseka okungcolile, ifanele i-PCR/qPCR, i-NGS, nezinye izinhlelo zokusebenza zebhayoloji yamangqamuzana. Lapho ihlanganiswe neI-BigfishI-nucleic acid extractor enobuhlalu obuzibuthe, ifaneleka kahle ukukhishwa okuzenzakalelayo kwamavolumu amakhulu esampula.
Izici Zomkhiqizo:
Ukusebenziseka Kwesampula Okubanzi: Kufanelekile ukukhishwa kwe-nucleic acid emithonjeni ehlukahlukene ye-viral DNA/RNA, okuhlanganisa i-HCV, i-HBV, i-HIV, i-HPV, namagciwane e-pathogenic ezilwane.
Kuyashesha futhi Kulula: Ukusebenza okulula okudinga kuphela isampula yokwengezwa ngaphambi kokucubungula umshini, ukususa isidingo sezinyathelo eziningi ze-centrifugation.Ihambisana nama-extractors e-nucleic acid azinikele, afaneleka ngokukhethekile ukucubungula isampula ephezulu.
Ukunemba Okuphezulu: Isistimu ye-buffer eyingqayizivele iqinisekisa ukukhiqiza kabusha okuhle kakhulu lapho ikhipha amasampula egciwane agxile kancane.
Izinsimbi Ezihambisanayo:
I-Bigfish Sequence BFEX-32E/BFEX-32/BFEX-96E
Isikhathi sokuthumela: Sep-04-2025
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