Ungayikhetha kanjani i-cyper efanelekile ye-thermal yezidingo zakho zocwaningo

Amakhilomitha ashisayoamathuluzi asemqoka uma kukhulunywa nge-biology yamangqamuzana kanye nocwaningo lwezakhi zofuzo. Eyaziwa nangokuthi umshini we-PCR (Polymerase Chain Reaction), le divayisi ibalulekile ukuze ikhulise i-DNA, okwenza kube yitshe lezicelo zohlelo lokusebenza oluhlukahlukene kufaka phakathi ukuhlaziya, ukuhlela kanye nokuhlaziywa kwenkulumo. Kodwa-ke, kunezinketho eziningi kakhulu emakethe ezikhetha i-cyper eyi-thermal efanelekile yezidingo zakho zocwaningo zingaba umsebenzi owesabekayo. Nazi ezinye zezinto ezibalulekile okufanele uzicabangele lapho wenza ukukhetha kwakho.

1. Qondisisa izidingo zakho zocwaningo

Ngaphambi kokungena ekutholeni amakhiloshi ahlukene ama-Thermal, kubalulekile ukuhlola izidingo zakho ezithile zocwaningo. Cabanga ngohlobo lokuhlola ozoluqhuba. Ingabe usebenzisa i-PCR ejwayelekile, i-PCR ye-PCR (QPCR), noma uhlelo lokusebenza lokuphamba oluphezulu? Ngayinye yalezi zinhlelo zokusebenza zingadinga izici ezahlukahlukene namakhono e-cyper ye-thermal.

2. Uhla lokushisa kanye nokufana

Uhla lokushisa lwe-cyper ye-thermal luyinto ebucayi. Amaphrothokholi amaningi we-PCR adinga igxathu lokuchazwa cishe ngo-94-98 ° C, isinyathelo sokungenzi-annealing ngo-50-65 ° C, nesinyathelo sokunwetshwa ngo-72 ° C. Qinisekisa ukuthi i-cyper eshisayo oyikhethayo ingaphatha la mazinga okushisa nokuthi izinga lokushisa lisatshalaliswa ngokulinganayo kuyo yonke imodyuli. Ukufana kokushisa okungafanele kungathinta ucwaningo lwakho ngokudala imiphumela engahambisani.

3. Ifomethi ye-block kanye namandla

Abakhi be-Thermal beza ngamafomethi ahlukahlukene ahlukahlukene, kufaka phakathi amapuleti angu-96, amapuleti amahle ka-384, kanye namapuleti amahle ka-1536. Ukukhetha kwefomethi ye-block kufanele kuhambisane nezidingo zakho zokusebenzisa. Uma wenza izivivinyo eziphikisana kakhulu, ungadinga ifomethi enkulu yebhlokhi. Ngakolunye uhlangothi, ngokuhlolwa okuncane okunokulinganiselwa, kungase kufinyeleleke kahle ipuleti engu-96. Ngokwengeziwe, cabanga ukuthi ngabe udinga amamojula aguqukayo ezifomeni ezihlukile, ngoba lokhu kungakhulisa ukuguquguquka kocwaningo lwakho.

4. Isivinini nokusebenza kahle

Esimweni sanamuhla sokucwaninga ngesivinini esisheshayo, isikhathi singesisekelo. Funa i-cyper eshisayo ngokushisa okusheshayo namakhono okupholisa. Amanye amamodeli athuthukile angaqedela umjikelezo we-PCR ngaphansi kwemizuzu engama-30, asheshise kakhulu ukuhamba kwakho komsebenzi. Ngaphezu kwalokho, izici ezifana nemodi esheshayo noma amazinga okushisa asheshayo akhulisa ukusebenza kahle, okukuvumela ukuthi ucubungule amasampula amaningi ngesikhathi esincane.

5. Isikhombimsebenzisi somsebenzisi nesoftware

Isibonisi esisebenziseka kalula sibalulekile ekusebenzeni kahle. Funa i-cyper eshisayo ngesikrini esinembile sokuthinta, izinketho ezilula zokuhlela, nezinqubomgomo ezisethiwe. Amamodeli athuthukile angafika nesoftware evumela ukuqapha kwangempela kanye nokuhlaziywa kwedatha, okuzuzisa kakhulu izinhlelo zokusebenza ze-QPCR. Qiniseka ukuthi isoftware iyahambisana nezinhlelo zakho ezikhona futhi ingakwazi ukuphatha okukhipha idatha oyidingayo.

6. Ukucatshangelwa kwesabelomali

Ama-cyclers ashisayo ahlukahluka kakhulu ngentengo, ngakho-ke kubalulekile ukuba nesabelomali ngaphambi kokuqala ukuthenga eyodwa. Ngenkathi kungahle kulingeze ukuhamba nenketho eshibhile, cabanga ngenani lesikhathi eside lokutshala imali emshinini osezingeni eliphezulu ohlangabezana nezidingo zakho zocwaningo. Cabanga hhayi ngentengo yokuqala yokuthenga, kodwa futhi nezindleko zokudla, ukugcinwa, kanye nokuthuthukiswa okungenzeka.

7. Ukuxhaswa Komkhiqizi Newaranti

Ekugcineni, cabanga ngezinga lokusekelwa newaranti enikezwe ngumenzi. I-cypler ethembekile ye-thermal kufanele inikeze iwaranti ephelele futhi inokusekelwa kwamakhasimende kokuxazulula inkinga nokulungisa. Lokhu kusindisa isikhathi nezinsizakusebenza ngokuhamba kwesikhathi.

Ekuphetheni

Ukukhetha okulungileI-Thermal CypeerUkuze izidingo zakho zocwaningo ziyisinqumo esibucayi esingathinta impumelelo yokuhlolwa kwakho. Ngokucabanga ngokucophelela izidingo zakho ezithile, uhla lokushisa, i-module formace, ijubane, isikhombimsebenzisi somsebenzisi, isabelomali, nokwesekwa komenzi, ungenza isinqumo esinolwazi esizothuthukisa imiphumela ethembekile. Isikhathi sokutshala kule nqubo yokukhetha sizokhokha ekugcineni kwikhwalithi kanye nokusebenza komsebenzi wakho wesayensi.