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I-reagent entsha yokubona isifo sezingulube i-freeze-drying evela ku-Big Fish yethuliwe. Ngokungafani nama-reagents avamile okuthola uketshezi adinga ukulungiswa mathupha kwezinhlelo zokusabela, lesi siphehli sithatha ifomu le-microsphere elixutshwe ngokuphelele elifakwe ngaphambili eliqandisiwe, elingagcinwa ekamelweni lokushisa ngaphandle kokuthikameze ukusebenza kwe-reagent. Ngesikhathi sokutholwa, i-nucleic acid ekhishiwe kuphela edinga ukwengezwa ngokuvula isivalo. Ngemuva kokuthi i-reagent ihlakazwe ngokuphelele, ingahlolwa emshinini. Imataniswe nama-reagents akhipha i-nucleic acid azenzakalelayo ngokuphelele kanye nemikhiqizo yezinsimbi evela ku-Big Fish, i-Big Fish yethule ngokusemthethweni isixazululo sokutholwa ngokushesha semizuzu engama-40 sezifo zezingulube. Ngamaphrojekthi amakhulu ayisithupha okutholwa okuhlanganisa i-Blue Ear, i-Pseudorabies, i-Swine Fever, i-Circovirus, i-Non Circovirus, kanye ne-Porcine Influenza, kuthatha cishe imizuzu engu-40 kuphela ukuqedela yonke inqubo yokutholwa komthamo we-PCR fluorescence kusukela ekucubungulweni kwesampula kuya emiphumeleni yokutholwa.
Inqubo Yesixazululo
1. Ukukhipha okusebenzayo - amasampula amaningi angacutshungulwa emizuzwini eyi-10
Ngokusebenzisa i-Big Fish universal nucleic acid extraction kanye ne-reagent yokuhlanza ehlanganiswe nethuluzi lokuhlanza i-nucleic acid ezenzakalela ngokugcwele ngokugcwele, ukukhipha i-nucleic acid kungenziwa kumasampula ahlukahlukene (okuhlanganisa igazi eliphelele, i-serum, i-plasma, i-swabs yemvelo, i-oral swabs, i-fecal swabs, njll.) phakathi nemizuzu eyi-10 ngaphandle kwesidingo sesampula esiyinkimbinkimbi sangaphambi kokwelashwa. Ngemuva kokulayisha isampula, ingakhishwa emshinini.
2. Ukukhulisa ngokushesha -30 min ukulinganisa kwe-fluorescence okusheshayo
Ngokusebenzisa i-Big Fish BFOP-1650 Fluorescence Quantitative PCR Analyzer, imodi yasendle ye-Big Fish frize okomisiwe okutholwayo kungenziwa isebenze. Inhlanganisela yama-reagents aqandayo kanye nohlelo lokuhlonza ngokushesha lwemizuzu engama-30 lungafinyelela ngempela isivalo esivulekile nokuhlola endaweni.
3. Ukuhlaziya okuhlakaniphile - ukusebenza okubalulekile, ukuhlaziya okuzenzakalelayo
I-Big Fish BFOP-1650 fluorescence quantitative PCR analyzer ayidingi izilungiselelo zohlelo eziyinkimbinkimbi. Chofoza okuthi "Qala" ukuze zonke izinto ezitholwayo ziqale ukutholwa. Ngemva kokuphothulwa kwe-amplification, yenza ngokuzenzakalelayo ukwahlulela okuhle nokubi ngaphandle kokuhlaziywa kwedatha okwenziwa ngesandla.
Isikhathi sokuthumela: Jun-21-2025
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