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I-reagent entsha yokuthola ukomisa isifo sengulube evela ku-Big Fish isiqalisiwe. Ngokungafani nama-reagent endabuko okuthola uketshezi adinga ukulungiswa ngesandla kwezinhlelo zokusabela, le reagent isebenzisa ifomu le-microsphere elihlanganisiwe ngokuphelele, elingagcinwa ekamelweni lokushisa ngaphandle kokuthinta ukusebenza kwe-reagent. Ngesikhathi sokutholwa, i-nucleic acid ekhishwe kuphela okudingeka yengezwe ngokuvula isivalo. Ngemva kokuba i-reagent isincibilike ngokuphelele, ingahlolwa emshinini. Ihlanganiswe nama-reagent okukhipha i-nucleic acid ezenzakalelayo ngokuphelele kanye nemikhiqizo yezinsimbi ezivela ku-Big Fish, i-Big Fish yethule ngokusemthethweni ikhambi lokuthola ngokushesha lemizuzu engama-40 lezifo zengulube. Ngamaphrojekthi ayisithupha amakhulu okuthola afaka phakathi i-Blue Ear, i-Pseudorabies, i-Swine Fever, i-Circovirus, i-Non Circovirus, kanye ne-Porcine Influenza, kuthatha imizuzu engaba ngu-40 kuphela ukuqeda yonke inqubo yokutholwa kwe-PCR fluorescence quantitative kusukela ekucutshungulweni kwesampula kuya emiphumeleni yokutholwa.
Inqubo Yesixazululo
1. Ukukhipha okusebenzayo - amasampula amaningi angacutshungulwa ngemizuzu eyi-10
Ngokusebenzisa i-Big Fish universal nucleic acid extraction and purification reagent ehlanganiswe nethuluzi lokukhipha nokuhlanza i-nucleic acid elizenzakalelayo ngokuphelele, ukukhishwa kwe-nucleic acid kungenziwa kumasampula ahlukahlukene (kufaka phakathi igazi eliphelele, i-serum, i-plasma, ama-environmental swabs, ama-oral swabs, ama-fecal swabs, njll.) kungakapheli imizuzu eyi-10 ngaphandle kwesidingo sokwelashwa kwesampula okuyinkimbinkimbi. Ngemva kokulayisha isampula, ingakhishwa emshinini.
2. Ukukhulisa okusheshayo - isilinganiso se-fluorescence esheshayo semizuzu engama-30
Ngokusebenzisa i-Big Fish BFOP-1650 Fluorescence Quantitative PCR Analyzer, imodi yasendle ye-Big Fish freeze dry detection reagent ingasetshenziswa. Ukuhlanganiswa kwama-reagent okomisa ngokuqandisa kanye nohlelo lokuthola ngokushesha lwemizuzu engama-30 kungafeza ngempela isivalo esivulekile kanye nokuhlolwa kwasendaweni.
3. Ukuhlaziywa okuhlakaniphile - ukusebenza kokhiye, ukuhlaziywa okuzenzakalelayo
I-Big Fish BFOP-1650 fluorescence quantitative PCR analyzer ayidingi izilungiselelo zohlelo eziyinkimbinkimbi. Chofoza okuthi "Qala" ukuze zonke izinto zokuthola ziqale ukutholwa. Ngemva kokuthi ukukhuliswa sekuqediwe, yenza ngokuzenzakalelayo ukwahlulela okuhle nokubi ngaphandle kokuhlaziywa kwedatha ngesandla.
Isikhathi sokuthunyelwe: Juni-21-2025
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