Iphrothokholi yokuthola imvelaphi yesilwane se-Bigfish

Inkinga yokuphepha kokudla iya ngokuya iba nkulu. Njengoba umehluko wentengo yenyama ukhula kancane kancane, isigameko "sokulengiswa kwekhanda lemvu nokuthengisa inyama yenja" kwenzeka njalo. Okusolwa ngokukhwabanisa kwenkulumo-ze engamanga kanye nokwephulwa kwamalungelo asemthethweni nezintshisekelo zabathengi, kunciphisa isithunzi somphakathi sokuphepha kokudla, okuholela kumthelela omubi emphakathini. Ukuze kuqinisekiswe kangcono ukuphepha kokudla kanye nokukhiqizwa kokuphepha kokufuywa kwemfuyo ezweni lethu, izindinganiso nezindlela zokuhlola ezithembekile zidingeka ngokushesha.

Ngokuqamba okusha okuqhubekayo nokuphikelela kwabacwaningi, i-Bigfish ithuthukise ngokuzimela ikhithi yokuthola etholakala ezilwaneni, ihlinzeka ngezixazululo ezithuthuke kakhulu nezisheshayo kumakhasimende ethu! Sizizwa sihloniphekile futhi ngokukwazi ukusiza amakhasimende ethu ukuxazulula izinkinga zawo.
Igama lomkhiqizo: Ikhithi yokuthola imvelaphi yezilwane (ingulube, inkukhu, ihhashi, inkomo, imvu)
Ukuzwela okuphezulu: ubuncane bomkhawulo wokutholwa ngu-0.1%
Ukucaciswa okuphezulu: ukuhlonza okunembile kwazo zonke izinhlobo "zenyama yangempela nenkohliso", akukho ukusabela okuphambene
1. Isampula yokucubungula
Amasampula ahlanjululwe kabili kuya kathathu nge-ethanol engu-70% namanzi acwecwe kabili, aqoqwe kumashubhu e-centrifuge angu-50 ml noma izikhwama ezivaliwe ezihlanzekile futhi agcinwe eqandisiwe ku -20 °C. Amasampula ahlukaniswe aba izingxenye ezintathu ezilinganayo, okuhlanganisa isampula okufanele lihlolwe, isampula elihloliwe kabusha kanye nesampula eligciniwe.
2. Ukukhishwa kwe-nucleic acid
Amasampula ezicubu ayomiswa futhi agaywe kahle noma engezwe ku-nitrogen ewuketshezi, bese impushana odakeni nasetsheni, bese i-DNA yezilwane ikhishwa kusetshenziswa i-automatic.i-nucleic acid extractor + I-Magpure Animal Tissue Genomic DNA Purification Kit.

(Isethi yokukhishwa kwelabhorethri)

3. Ukuhlolwa kokukhulisa amandla
Ukuhlolwa kokukhulisa ulwazi kwenziwa kusetshenziswa i-Bigfish sequential real-time quantitative quantitative PCR analyzer + ikhithi yokuthola esuselwa esilwaneni ukuze kutholwe ngokunembile ukuthi inyama yonakele yini ngokuya ngemiphumela engemihle, ukuvikela kangcono amalungelo abathengi nokuphepha kokudla.

Izibonelo: Ikhithi Yokuhlola Imvelaphi Yezilwane (Izimvu)