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Inkinga yokuphepha kokudla iya ngokuya imbi kakhulu. Njengoba umehluko wentengo yenyama unwebeka kancane kancane, isehlakalo 'sokulengisa ikhanda lezimvu nokuthengisa inyama yenja "kwenzeka njalo. Kusolwa ukuthi inkohliso yenkulumo-ze nokwephulwa kwamalungelo nezintshisakalo ezisemthethweni zabathengi, yehlisa idumela lomphakathi lokuphepha kokudla, okuholela ekuphumeni komthelela omubi. Ukuze uqinisekise kangcono ukuphepha kokudla kanye nokukhiqizwa kokuphepha komlimi wemfuyo ezweni lethu, izindinganiso zokuhlola ezithembekile kanye nezindlela zidingeka ngokuphuthumayo.
Ngokuqamba okuqhubekayo nokuphikelela kwabaphenyi, iBigfish ithuthukise ngokuzimela ikhithi yokutholwa kwezilwane, ukuhlinzeka ngezixazululo ezithuthukile nezisheshayo zamakhasimende ethu! Sizizwa sihlonishwa kakhulu ukukwazi ukusiza amakhasimende ethu ukuxazulula izinkinga zazo.
Igama Lomkhiqizo: Ikhithi yokuthola izilwane yasekuqaleni (ingulube, inkukhu, ihhashi, inkomo, izimvu)
Ukuzwela okuphezulu: Umkhawulo wokutholwa okuncane okungu-0,1%
Ukucaciswa okuphezulu: Ukukhonjwa okunembile kwazo zonke izinhlobo zenyama "yangempela neyengembango", akukho ukuqhubeka kokusebenza
1, ukucubungula isampula
Amasampula akhanyiswe kabili kuya kathathu nge-70% ethanol namanzi afakwe kabili, aqoqwe kumashubhu ahlanzekile ahlanzekile we-ML centrifuge noma ahlanzekile efrijini ku-20 ° C. Amasampula ahlukaniswe izingxenye ezintathu ezilinganayo, kubandakanya nesampula elizohlolwa, isampula elibuyiselwe kanye nesampula egciniwe.
2, ukukhishwa kwe-nucleic acid
Amasampula wezicubu ezomile nomhlabathi kahle noma angezwe ku-nitrogen ewuketshezi, bese kuba yimpuphu noma udaka ngo-pestle, kanye ne-animal genomic DNA kukhishwe kusetshenziswa okuzenzakalelayoI-nucleic acid extractor + I-Magpire Animal Tempis genomic DNA HINISH.
(Isethi yokukhishwa kwelebhu)
3. Ukuhlolwa kwe-Amplification
Ukuhlolwa kwe-Amplification kwenziwa kusetshenziswa i-bigfish real-time fluorescence pcr analyzer + ikhithi yokutholwa kwezilwane ukunquma ngokunembile ukuthi inyama isongelwe ngokusho kwemiphumela emibi, ukuvikela kangcono amalungelo abathengi nokuphepha kokudla.
| Igama lomkhiqizo | Into No. | ||
|
Into yokwenza utho | I-othomathikhi ye-nucleic acid extractor | BFEX-32/96 | |
| Ithuluzi le-PRCRITITE ye-PRCRITION yesikhathi sangempela (48) | BFQP-48 | ||
|
Isiphendukeleko | Izicubu zezilwane zezicubu ze-genomic dna kit | BFMP01R / BFMP01R96 | |
| I-Animal Origim Test Kit (Bovine) | Bfrett13m | ||
| I-Animal Origim Test Kit (Sheep) | Bfrett14m | ||
| I-Animal Origim Test Kit (ihhashi) | Bfrett15m | ||
| I-Animal Origim Test Kit (Swine) | I-Bfrett16m | ||
| I-Animal Origim Test Kit (Inkukhu) | Bfrett17m | ||
| -Khoshwa
| 96 Deep Well Plate 2.2ml | BFMH01 / BFMH07 | |
| I-Magnetic Rod Set | Bfmh02 / bfmh08 | ||
Izibonelo: Ikhithi yokuhlola yezilwane (izimvu)
Isikhathi sePosi: Nov-23-2022
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