Ukuguqula ukukhishwa kwe-nucleic acid: ithuluzi lokugcina le-molecular biology lagerator

Emkhakheni we-biology molecular, ukukhishwa kwama-nucleic acid kuyinqubo eyisisekelo eyakha isisekelo sohlobo oluthile lokuhlaziywa kofuzo nolofuzo. Ukusebenza kahle kanye nokunemba kokukhishwa kwe-nucleic acid kubalulekile empumelelweni yezicelo ezaphansi ze-Downstream ezifana ne-PCR, ukulandelana nokuhlolwa kofuzo. Njengobuchwepheshe buthuthuka, ama-laboratoratories ayaqhubeka nokufuna amathuluzi amasha alula futhi athuthukise inqubo yokukhipha. Yilapho kungena khona i-nucleic acid extractor, ukuguqula indlela ama-nucleic acid akhishwe futhi abeke amazinga amasha we-molecular biology.

Le khasiI-nucleic acid extractorInomklamo wobuhlakani onobuhlakani futhi ifaka imisebenzi yokusika - imisebenzi yokusika ukuze ihlangabezane nezidingo ezithile zokuhlolwa kofuzo kanye nokucwaninga ngezihloko. Eminye yemisebenzi yayo eyinhloko ukulawula okungcolisayo kwe-UV, ukuqinisekisa ubumsulwa bama-nucleic acid akhishwe ngokunciphisa ubungozi bokungcoliswa kwangaphandle. Lokhu kubaluleke kakhulu ezilungiselelweni zomtholampilo lapho ubuqotho bezezimali lubalulekile. Ngaphezu kwalokho, umsebenzi wokushisa insimbi uhlinzeka ngokulawulwa kokushisa okuqondile ukufeza izimo ezifanele ngesikhathi senqubo yokukhipha.

I-nucleic acid extractor ibuye iza nesibonisi esikhulu sesikrini sokuthinta, okwenza kube nobungane futhi isebenzise ukusebenza. Lokhu akugcini nje ngokwenza inqubo yokwenza inqubo yokukhipha, futhi kunciphisa amathuba okuba yiphutha lomuntu, ukuqinisekisa imiphumela engaguquki nethembekile. Ukusebenziseka lula nokusebenza kahle okunikezwa isikhombimsebenzisi sokuthinta yenza leli thuluzi litholakale kubo bobabili abacwaningi abanolwazi nalabo abasha emkhakheni we-biology molefanelar.

Ngaphezu kwalokho, i-nucleic acid extractor iyithuluzi elinamandla elingahlangabezana nezidingo ezahlukahlukene ze-molecular biology gameratories. Ukuguquguquka kwayo kuvumela ukukhishwa kwama-nucleic acid kusuka ezinhlotsheni ezahlukahlukene zesampula, kufaka phakathi igazi, izicubu kanye namangqamuzana amasiko. Lokhu kuvumelana nalokhu kwenza kube yimpahla ebaluleke kakhulu ezinhlobonhlobo zezicelo, ekuhlolweni kwemitholampilo ukuze ucwaninge umsebenzi.

Emkhakheni wokuhlolwa kofuzo lwezempilo, amathuluzi okukhishwa kwe-nucleic acid adlala indima ebalulekile ekuhlaziyeni ngokushesha futhi ngokunembile okumaka izakhi zofuzo kanye nokuguqulwa kwezakhi zofuzo. Amandla awo okukhipha ama-nucleic acid asezingeni eliphakeme kusuka kumasampula emitholampilo aqinisekisa ukwethenjwa kokuhlolwa kokuxilonga futhi azungeze indlela yomuthi owenziwe ngezifiso. Ngaphezu kwalokho, ekucwaningweni kwesifundo kuma-molecular biology gameratories, ithuluzi lisiza ukuhlola ukwahlukahluka kofuzo kanye nokucaciselela izindlela ze-molecular zezinto eziphilayo.

Ekuphetheni, ithuluzi le-nucleic acid extraction limelela i-paradigm shift emkhakheni wokukhishwa kwe-nucleic acid. Idizayini yayo entsha, ukulawulwa kokungcolisa i-UV, amakhono okushisa, kanye nesibonisi esisebenziseka kalula kwenza kube ngumguquki womdlalo wama-molefanelar biology gameratories. Ngokwenza lula inqubo yokukhipha nokuqinisekisa ubuqotho bama-nucleic acid, ithuluzi linika amandla abaphenyi kanye nodokotela ukuthi banciphise ubunzima bezakhi zofuzo kanye ne-genomics. Njengoba ubuchwepheshe buqhubeka buvela,Ukukhishwa kwe-nucleic acidAmathuluzi angaphambili, eqhubekela phambili ngokushayela ekuhlaziyeni kofuzo nocwaningo lwamamolekisi.