Ukuguqula i-PCR: I-FastCycler Cymer eshisayo

Emkhakheni we-biology molecular,amakhilomitha ashisayo iyithuluzi elisemqoka ekuphenduleni kwe-polymerase chain reaction (PCR). Njengabaphenyi kanye nama-laboratories aphishekela ukusebenza kahle nokunemba, i-FastCyCler isiphenduke umshini womdlalo emkhakheni. Ngobuchwepheshe bayo obunqenqemeni kanye nokusebenza okuphezulu, i-FastCycteer ibeka indinganiso entsha yokuhamba ngebhayisekili eshisayo.

I-FastCycler inikwa amandla yizinto ezisezingeni eliphakeme ze-Pellier ezivela kumaMarlow, e-USA. Lezi zinto zenzelwe ngokucophelela ukuhlinzeka ngokulawulwa okungcono kakhulu kwezinga lokushisa, ngamanani okushisa afinyelela ku-6 ° C / s. Le amandla asheshayo e-Rap ihlisa kakhulu isikhathi esidingekayo kumjikelezo ngamunye we-PCR, ukuvumela abaphenyi ukuthi bagcwalise izivivinyo ngokushesha ngaphandle kokuyekethisa ikhwalithi.

Enye yezinto zokusukuma ze-FastCycler ziyisibalo sayo somjikelezo esihlaba umxhwele, edlula imijikelezo eyizigidi eziyi-100. Lokhu kuqina kuqinisekisa ukuthi amalebhu angasebenzisa i-fastcycler isikhathi eside, okwenza kube ngukutshala imali okusebenzayo kunoma yisiphi isikhungo sokucwaninga. Impilo ende ye-FastCycler isho ukuthi abacwaningi bangagxila ekuhlolweni kwabo ngaphandle kokukhathazeka ngokuhluleka kwemishini noma isidingo sokubuyiselwa njalo.

Ukunemba okushisa kubalulekile ku-PCR, futhi i-FastCycler idlula kulokhu. Sisebenzisa ukufudumalo okuthuthukile kwe-thermoelectric kanye nobuchwepheshe obupholile, ehlanganiswe ne-PID (ngokulinganayo-okuhlanganisiwe-okususelwa) Ukulawulwa kokushisa, i-fastcycler igcina izinga eliphakeme lokushisa lokushisa kuyo yonke inqubo yokuhamba ngebhayisikile kuyo yonke inqubo yokuhamba ngebhayisikile. Lokhu kunemba kubalulekile ekufezeni imiphumela ethembekile neyakhiwa kabusha, okubalulekile ocwaningweni lwesayensi.

Ukufana kuyo yonke imithombo kungenye futhi isici esibucayi sokuhamba ngebhayisikile, futhi i-fastcycler ayidumazi. I-FastCyCler yakhelwe ukuqinisekisa iphrofayili yokushisa engaguquki kuyo yonke indawo ngayinye, inciphisa ubungozi bokuhlukahluka emiphumeleni ye-PCR. Lokhu kufana kubaluleke kakhulu lapho usebenza ngamasampula adinga ukumplizwa okuqondile, ngoba kusiza ukuqinisekisa ukuthi konke ukusabela kwenziwa ngaphansi kwezimo ezifanayo.

Ngaphezu kwalokho, i-fastcycler isebenza buthule, okwenza ilungele ama-labs adinga indawo yokusebenza ethule. Lesi sici sivumela abacwaningi ukuthi benze izivivinyo ngaphandle kokuphazanyiswa ngumshini womshini, benze umkhathi ogxile kakhulu futhi okhiqizayo.

Ngaphezu kokucaciswa kwayo kwezobuchwepheshe, i-fastcycler yakhelwe ngokusebenza kobungani emqondweni. Isimo sayo esibonakalayo kanye nezinketho ezilula zokuhlela zenza kube lula kubo bobabili abacwaningi abanolwazi nama-novices okufanele awasebenzise. Amandla okwenza ngezifiso ama-protocols futhi aqaphe kalula inqubekela phambili ethuthukisa ulwazi lomsebenzisi jikelele, ukuvumela ososayensi ukuthi bagxile ocwaningweni lwabo kunokuba basebenzise umshini oyinkimbinkimbi.

Ngokufingqa, i-fastcyclerI-Thermal Cypeerimele ukuthuthuka okukhulu kubuchwepheshe be-PCR. Ngezinto zayo ezisezingeni eliphakeme ze-Peltier, ukumiswa okusheshayo, inkomba enhle yokuhamba ngebhayisikile, kanye nokulawulwa kokushisa okuthuthukile, kunikeza ukusebenza okungenakuqhathaniswa kwezicelo ze-molecular Biology. Ukuhlanganiswa kokunemba, ukufana, kanye nokusebenza komsindo ophansi kwenza i-FastCycler ilithuluzi elibalulekile kunoma yikuphi ilabhorethri okuhlose ukuthola kahle imiphumela esezingeni eliphakeme. Njengoba isidingo se-PCR esisheshayo nesinokwethenjelwa siyaqhubeka nokukhula, i-fastcycler ivelele njengomholi emkhakheni, okwenza abacwaningi bakwazi ukucindezela imingcele yokutholwa kwesayensi.