I-Revolutionizing PCR: I-FastCycler Thermal Cycler

Emkhakheni webhayoloji yamangqamuzana,amabhayisikili ashisayozingamathuluzi abalulekile kubacwaningi nakososayensi. Badlala indima ebalulekile enqubweni ye-polymerase chain reaction (PCR), eyisisekelo sokukhulisa i-DNA, i-cloning kanye nokuhlaziywa kwezakhi zofuzo okuhlukahlukene. Phakathi kwamabhayisikili amaningi ashisayo emakethe, i-FastCycler igqama ngezici zayo ezithuthukile nezinto ezisezingeni eliphezulu, iba yimodeli yokuqamba izinto ezintsha nokusebenza kahle.

Enhliziyweni ye-FastCycler ukuzibophezela kwayo kwikhwalithi, isebenzisa ama-elementi e-premium Peltier avela e-Marlow, e-USA. Lezi zici zaziwa ngokwethembeka nokusebenza kwazo, okuvumela i-FastCycler ukuthi ifinyelele amazinga okushisa amangalisayo afika ku-6°C/S. Leli khono lokugijima elisheshayo libalulekile ekwehliseni isamba sesikhathi esidingekayo sokuhamba ngebhayisikili kwe-PCR, okuvumela abacwaningi ukuthi bathole imiphumela ngokushesha ngaphandle kokwehliswa ubuqotho bokuhlolwa.

Esinye sezici ezivelele ze-FastCycler ukubalwa kwayo komjikelezo ohlaba umxhwele, okudlula imijikelezo eyizigidi eziyi-100. Lokhu kuqina kusho ukuthi abacwaningi bangasebenzisa i-FastCycler isikhathi eside, okuyenza ibe ukutshalwa kwezimali okungabizi kakhulu kumalabhorethri adinga ukuhamba ngebhayisikili okushisayo okuqhubekayo nokuphindaphindayo. Ukuphila isikhathi eside kwe-FastCycler kuwubufakazi bomklamo nobunjiniyela bayo obumangelengele, okuqinisekisa ukuthi ingakwazi ukumelana nobunzima bokusetshenziswa kwelabhorethri yansuku zonke.

Ukunemba kwezinga lokushisa kubalulekile ezinhlelweni ze-PCR, futhi i-FastCycler iyaphumelela kulokhu. Ngobuchwepheshe bokufudumeza okuthuthukisiwe kwe-thermoelectric kanye nokupholisa okuhlanganiswe ne-PID (i-proportional-integral-derivative) yokulawula izinga lokushisa, i-FastCycler igcina izinga eliphezulu lokunemba kwezinga lokushisa kuyo yonke inqubo yebhayisikili. Lokhu kunemba kubalulekile ukuze kuzuzwe ukukhuliswa okuphelele kwe-DNA, njengoba ngisho nokuchezuka okuncane kwezinga lokushisa kungaholela emiphumeleni emibi noma ukwehluleka kokuhlola.

Ukufana kuyo yonke imithombo kungenye isici esibalulekile sokuhamba ngebhayisikili okushisayo, futhi i-FastCycler ayidumazi. Idizayini yayo iqinisekisa ukuthi wonke amasampuli ayashisisa futhi apholiswe ngokuqhubekayo, okubalulekile ekuhloleni okudinga izimo ezifanayo. Lokhu kufana kunciphisa ukuhlukahluka kwemiphumela, kunikeza abacwaningi ithemba lokuthi idatha yabo inokwethenjelwa futhi iyakwazi ukukhiqizwa kabusha.

Ngaphezu kwalokho, i-FastCycler isebenza emazingeni aphansi omsindo, okwenza ilungele izindawo zelebhu ezidinga umoya onokuthula. Lesi sici asigcini nje ngokuthuthukisa izimo zokusebenza zabacwaningi, kodwa futhi senza ulwazi ngelebhu lugxile kakhulu futhi lusebenze kahle.

Kafushane, iI-FastCycler Thermal Cyclerimele intuthuko enkulu kubuchwepheshe be-PCR. Ngezinto zayo zekhwalithi ephezulu zePeltier, amanani erempu esheshayo, inkomba yebhayisikili enhle kakhulu, kanye nendlela yokulawula izinga lokushisa elithuthukisiwe, yakhelwe ukuhlangabezana nezidingo zocwaningo lwesimanje lwebhayoloji yamangqamuzana. Noma ngabe wenza ukukhulisa i-DNA okujwayelekile noma uhlanganyela ocwaningweni oluyinkimbinkimbi lwezakhi zofuzo, i-FastCycler iletha ukusebenza okuphezulu, ukwethembeka, nokusebenza kahle. Ukutshala imali ku-FastCycler kusho ukutshala imali esikhathini esizayo socwaningo lwakho, uqinisekisa ukuthi unamathuluzi owadingayo ukuze udlule imingcele yokutholwa kwesayensi.