Ukuguqula i-PCR: I-FastCycler Cymer eshisayo

Emkhakheni we-biology molecular,amakhilomitha ashisayobangamathuluzi asemqoka kubacwaningi nososayensi. Badlala indima ebalulekile kwinqubo ye-polymerase chain reaction (PCR), okuyisisekelo se-DNA Amplification, Cloning kanye nokuhlaziywa kofuzo okuhlukahlukene. Phakathi kwamabhayisekili amaningi afudumele emakethe, i-FastCycler ivelele ngezici zayo ezithuthukile kanye nezinto ezisezingeni eliphakeme, ziba yimodeli yokuqamba kanye nokusebenza kahle.

Enhliziyweni ye-FastCycler ukuzibophezela kwayo kwikhwalithi, kusetshenziswa izinto ze-Premium Peltic kusuka kuMarlow, e-USA. Lezi zinto zidume ngokuthembekile nokusebenza kwazo, zinika amandla i-fastcycler ukuze afinyelele amanani amangalisayo okushisa afinyelela ku-6 ° C / S. Lokhu kubheja okusheshayo kubalulekile ekunciphiseni isikhathi esiphelele esidingekayo sokuhamba ngebhayisikile se-PCR, okuvumela abaphenyi ukuthi bathole imiphumela ngokushesha ngaphandle kokuyekethisa ubuqotho bokuhlolwa.

Enye yezinto zokusukuma ze-FastCycler ziyisibalo sayo somjikelezo esihlaba umxhwele, edlula imijikelezo eyizigidi eziyi-100. Lokhu kuqina kusho ukuthi abacwaningi bangasebenzisa i-fastcycler isikhathi eside, okwenza kube ngukutshala imali okusebenzayo kwezindawo zokucwaninga ezidinga ukuhamba ngebhayisekili okuqhubekayo nokuphindaphindeka. Ukuphila isikhathi eside kwe-FastCycler kungukuvuselela umklamo wayo omatasa nobunjiniyela, ukuqinisekisa ukuthi kungamelana nobunzima bokusetshenziswa kwelebhu yansuku zonke.

Ukunemba okushisa kubalulekile kuhlelo lwe-PCR, futhi i-FastCycler idlula kulokhu. Ngokushisa kwe-thermoelectric okuthuthukile kanye nobuchwepheshe obupholile obuhlanganiswe ne-PID (ngokulinganayo-okuhlangene-okususelwa) Ukulawulwa kokushisa, i-FastCycte igcina izinga eliphakeme lokufakelwa kokushisa kuyo yonke inqubo yokuhamba ngebhayisikile kuyo yonke inqubo yokuhamba ngebhayisikile. Lokhu kunemba kubalulekile ekufezekiseni ukukhuliswa okuphelele kwe-DNA, njengoba ukuphambuka okuncane kokushisa kungaholela emiphumeleni engemihle noma ukwehluleka kokuhlola.

Ukufana kuyo yonke imithombo kungenye futhi isici esibucayi sokuhamba ngebhayisikile, futhi i-fastcycler ayidumazi. Idizayini yayo iqinisekisa ukuthi wonke amasampula ayafudumala futhi apholile ngokungaguquki, okubaluleke kakhulu ekuhlolweni okudinga izimo ezifanayo. Lokhu kufana kunciphisa ukuhlukahluka kwemiphumela, kunikeza abacwaningi ukuzethemba ukuthi idatha yabo ithembekile futhi iyavela kabusha.

Ngaphezu kwalokho, i-FastCycte isebenza emazingeni omsindo aphansi, okwenza kube kuhle ngezindawo ze-lab ezidinga umoya othule. Lesi sici asigcini nje ngokuthuthukisa izimo zokusebenza kwabacwaningi, kodwa futhi kwenza ukuthi lokho okuhlangenwe nakho kwelebhu kugxile kakhulu futhi kusebenze kahle.

Ngokufingqa,I-FastCycler Cymer eshisayoimele ukuthuthuka okukhulu kubuchwepheshe be-PCR. Ngezinto zayo ezisezingeni eliphakeme ze-Peltier, amanani ama-ramp asheshayo, inkomba yokuhamba kahle kwamabhayisekili, kanye nendlela yokulawula yokushisa ethuthukisiwe, yakhelwe ukufeza izimfuno zokucwaninga nge-biology molecular. Ukuthi wenzani ngokumelelwa kwe-DNA ejwayelekile noma uhlanganyele ocwaningweni oluyinkimbinkimbi lwafuzo, i-FastCycler iletha ukusebenza okuphezulu, ukuthembeka nokusebenza kahle. Ukutshala imali ku-fastcycler kusho ukutshala imali ngokuzayo kocwaningo lwakho, ukuqinisekisa ukuthi unamathuluzi owadingayo ukucindezela imingcele yokutholwa kwesayensi.