Ukuvela kwe-cyper ye-thermal: inguquko ku-DNA Amplification

Amakhilomitha ashisayoUbe yithuluzi elisemqoka labaphenyi nososayensi emikhakheni ye-molecular biology kanye nofuzo. Le divayisi entsha iguqule inqubo ye-DNA Amplification, okwenza isheshe, isebenze kahle, futhi inembe kakhudlwana kunangaphambili. Kule bhulogi, sizohlola ukuthuthukiswa kwabahamba ngamabhayisekili afudumele kanye nomthelela wabo emkhakheni we-biology molecular.

Umqondo wokuhamba ngebhayisikile, okubandakanya ukufudumeza okuphindaphindiwe futhi upholise inhlanganisela yokuphendula, uyisisekelo sokuphendula kwe-polymerase chain (PCR). I-PCR yindlela ekhulisa amakhophi angashadile noma ambalwa we-DNA ngama-oda amaningana ngobukhulu, ekhiqiza izinkulungwane eziya ezigidini zamakhophi alandelanayo. Ukuthuthukiswa kwama-cyplers afudumele kudlale indima ebalulekile ekusetshenzisweni okubanzi nasekuthuthukisweni kobuchwepheshe be-PCR.

Ama-cypler asolwandle aphambili ayenobuningi futhi adingekayo ukulungiswa kwamazinga okushisa kanye nokuqapha njalo. Kodwa-ke, njengoba ubuchwepheshe buthuthukile, amakhilogrendi anamuhla ashisayo asephenduke amathuluzi ayinkimbinkimbi angalawula ngokunembile lokushisa futhi afinyelele ezenzakalelayo. Lokhu kuthuthuka kukhuphule kakhulu isivinini kanye nokusebenza kahle kwe-DNA Amplification, okuvumela abaphenyi ukuthi benze i-PCR kalula futhi ngokuthembekile.

Enye yezinto ezintsha ezibalulekile ku-Thermal Cyper Technology kwakuwukuqalwa kwe-gcr ye-gradient PCR, okuvumela amazinga okushisa amaningi avelele ahlolwe ngasikhathi sinye ekuhlolweni okukodwa. Lesi sici sifakazele ukuthi siwusizo kakhulu ekwakheni izimo ze-PCR zethempulethi ethile ye-DNA, ukonga abacwaningi isikhathi nezinsizakusebenza.

Ngaphezu kwalokho, ukuhlanganiswa kwamandla we-PCR wesikhathi sangempela kuma-Thermal Cyplers kubuye kwandise ukusetshenziswa kwawo. I-Real-Time PCR, eyaziwa nangokuthi i-PCR ye-PCRITITION, iqapha ama-DNA Amplification ngesikhathi sangempela, ukuhlinzeka ngemininingwane ebalulekile kwisamba sokuqala sokulandelana kokulandelana kwelitshe. Lokhu kuguqule izindawo ezifana nokuhlaziywa kwe-gene short, i-genetyping, kanye nokutholwa kwe-pathogen.

I-miniaturiation yabakhi be-Thermal iphenduke umkhuba obalulekile eminyakeni yamuva, iqhutshwa isidingo sokuthwala kanye nokusebenza kahle. Lawa ma-Clampact, amakhilogremu aphathwayo atholakele athole izinhlelo zokusebenza ku-Field Research, Point-of-Care Diagstics, naseziseshini ezikhawulelwe kwizinsizakusebenza lapho ingqalasizinda yendabuko yezolabhothi ingase ishoda.

Ukubheka phambili, ikusasa leamakhilomitha ashisayoizobona izinto ezintsha kakhulu. Ubuchwepheshe obuqhamukayo obunjenge-digital PCR nezindlela zokukhulisa ama-isothermal zephula imingcele ye-DNA Amplification futhi inikeze amathuba amasha okutholwa okubucayi nokushesha kwe-nucleic acid ukutholwa.

Ngokufingqa, ukuthuthukiswa kwama-cyplers afudumele kuye kwaba nomthelela omkhulu emkhakheni we-biology molecular, ukuthuthuka okuqhubekayo ocwaningweni, ekuxilongeni, ne-biotechnology. Ukusuka emabhulokini okushisa okuqala okufudumele kumathuluzi athuthukisiwe athuthukile athuthukisiwe, abashayeli bama-Thermal baguqule ukuthuthukiswa kwe-DNA, okwenza kube lula ukuyisebenzisa futhi kuthembeke kakhulu kunangaphambili. Njengoba ubuchwepheshe buqhubeka buqhubekisa phambili, indima yabakhi be-Thermal ekubumbeni ikusasa le-biology molecular iqinisekile ukuthi ihlala ibalulekile.