Indima ebalulekile ye-nucleic acid expology ku-biotechnology yanamuhla

Emkhakheni okhula ngokushesha we-biotechnology, ukukhishwa kwama-nucleic acid (DNA ne-RNA) sekuyinqubo eyisisekelo yezinhlelo zokusebenza ezisukela ocwaningweni lwezakhi zofuzo. Enhliziyweni yale nqubo yi-nucleic acid extractor, ithuluzi elibalulekile elenza ukwehlukaniswa kwalawa ma-biomolecule asemqoka avela ezinhlobonhlobo zamasampula afanayo. Kule bhulogi, sizohlola ukubaluleka kwezindleko ze-nucleic acid, ukuthi zisebenza kanjani, kanye nomthelela wazo ocwaningweni lwesayensi nasentuthukweni yezempilo.

Ukuqonda ama-nucleic acid

Ama-nucleic acid yizindawo zokwakha zokuphila, ephethe imininingwane yofuzo edingekayo ekukhuleni, ekuthuthukisweni nasekusebenzeni kwazo zonke izidalwa. I-DNA (Deoxyribonucleic acid) yi-blueprint yetholo yezakhi zofuzo, kuyilapho i-RNA (Rionucleic acid) idlala indima ebalulekile ekuhumusheni imininingwane yofuzo ngamaprotheni. Amandla okukhipha futhi ahlaziye ama-nucleic acid abalulekile ezifundweni eziningi zesayensi ezinjenge-genomics, ama-traphscriptics kanye nokuxilonga ama-molecular.

Ukubaluleka kokukhishwa kwe-nucleic acid

Ukukhishwa kwe-nucleic acid kuyisinyathelo esibucayi ezinkundleni eziningi zelebhu. Noma ngabe isetshenziselwa ukuhlanganisa, ukulandelana noma ukuhlaziya okuchazayo kwe-gene, ikhwalithi nobumsulwa bama-nucleic acid angathinta kakhulu imiphumela yokuhlola. Izindlela zokukhishwa kwendabuko, njengokukhishwa kwe-phenol-chloroform noma ukuhanjiswa kotshwala, kungasebenza kakhulu futhi kudla isikhathi, futhi kuvame ukuholela emiphumeleni engahambisani. Yilapho amathuluzi okukhishwa kwe-nucleic acid angena.

Ukusebenza komgomo wensimbi yokukhishwa kwe-nucleic acid

Ama-nucleic acid extractorsSebenzisa amasu ahlukahlukene ukuhlukanisa i-DNA ne-RNA kusuka kumaseli nezicubu. Iningi langaphandle lanamuhla lisebenzisa izinhlelo ezizenzakalelayo ezihlanganisa izinyathelo ezimbalwa zenqubo yokukhipha, kufaka phakathi i-cellylis, ukuhlanzwa, kanye nokuqedwa. Lezi zinhlelo ngokuvamile zisebenzisa amakholomu asuselwa ku-silIca noma ubuhlalu bakazibuthe bokukhetha ukubopha ama-nucleic acid, ngaleyo ndlela kususe ukungcola okufana namaprotheni nama-lipid.

I-automation of nucleic acid extraction ingathuthuki nje kuphela ukusebenza kahle kepha futhi inciphisa ubungozi bephutha lomuntu, okuphumela emiphumeleni engaguquki futhi evuselelwayo. Ngaphezu kwalokho, amathuluzi amaningi okukhishwa kwe-nucleic acid aklanyelwe ukucubungula amasampula amaningi ngasikhathi sinye, abenze balungele izinhlelo zokusebenza ezisebenzisa kakhulu ekucwaningeni nasezilungiselelweni zemitholampilo.

Izicelo zocwaningo nezokwelapha

Izicelo ze-nucleic acid extractors zibanzi futhi zihlukahlukene. Ezitolo zokucwaninga, abakwa-nucleic acid arentractors bangamathuluzi asemqoka kuChywomic Research, okwenza ososayensi bahlaziye ukwahlukahluka kofuzo, umsebenzi wofuzo wokufunda, futhi uhlole ubudlelwano bemvelo. Ezimweni zemitholampilo, ukukhishwa kwe-nucleic acid kubalulekile ekuhlolweni kwezifo ezithathelwanayo, izifo zofuzo nomdlavuza. Amandla okukhipha ngokushesha nangokunembile ama-acid ama-nucleic kusuka kumasampula wesiguli avumela izinqumo zokwelashwa ezifika ngesikhathi nangokufanele.

Ngaphezu kwalokho, ukukhuphuka kwemithi eqondene nomuntu uqobo kuqokomise ukubaluleka kwezindleko ze-nucleic acid. Njengoba izindlela zokwelapha ezibhekiswe kabanzi zihambisana nokwakheka kofuzo komuntu ngamunye zivela, isidingo sezindleko ezisezingeni eliphakeme ze-nucleic acid sizoqhubeka nokukhula.

Ekuphetheni

Ngokufigqiwe,ama-nucleic acid extractorsamathuluzi abalulekile emkhakheni we-biotechnology, asiza ekunciphiseni kahle nangokwethembekile i-DNA ne-RNA kusuka kumasampula ahlukahlukene. Umthelela wabo ocwaningweni nasekuhlolweni kwemitholampilo ngeke kubhekwe ngokweqile, njengoba bevumela ososayensi nabezempilo ukuthi bavule izimfihlo ze-genome futhi bathuthukise imiphumela yeziguli. Njengoba ubuchwepheshe buqhubeka buqhubekisela phambili, singalindela ukuthi ama-extactors e-nucleic acid ukuze aqhubeke nokuvela, athuthukise amanye amakhono abo kanye nezinhlelo zokusebenza kwisayensi yempilo. Noma ngabe ungumcwaningi, umtholampilo, noma i-Science yabathandi, ukuqonda iqhaza labakwa-nucleic acid kuyisihluthulelo sokwazisa inqubekela phambili emangalisayo enziwa emkhakheni we-biotechnology.