Ukuvula amandla amakhilogremu ashisayo: ithuluzi elibalulekile le-biotechnology yanamuhla

Emikhakheni ye-biology ye-molecular kanye ne-biotechnology, ama-cyplers ashisayo ayizinsimbi ezisemqoka. Imvamisa ukubizwa ngomshini we-PCR, le mishini idlala indima ebalulekile ekukhuliseni i-DNA, okwenza kube yitshe lokucwaninga ngezinto zofuzo, ukuxilonga, kanye nezicelo ezahlukahlukene kwezokwelapha nezolimo. Ukuqonda umsebenzi nokubaluleka kwabakhi be-Thermal kungakhanyisela umthelela wabo entuthukweni yesayensi.

Yini i-cyper eshisayo?

A I-Thermal CypeerIngabe idivaysi yelabhoratri esebenza ngenqubo ye-polymerase chain reaction (PCR). I-PCR yindlela esetshenziselwa ukukhulisa izingxenye ezithile ze-DNA, okuvumela abaphenyi ukuthi bakhiqize izigidi zamakhophi okulandelana okuthile. Lokhu kukhuliswa kubalulekile ukuthola izinhlelo ezahlukahlukene, kufaka phakathi ukuhlaziya, ukucubungula kwe-gene deprion, kanye nokufakwa kweminwe yofuzo.
Abakhi be-Thermal basebenza ngochungechunge lwezinguquko zokushisa, ezibalulekile ezigabeni ezahlukahlukene ze-PCR. Lezi zigaba zibandakanya ukudonswa, ukunxusa, nokubumbana. Ngesikhathi se-denaturation, i-DNA esakhiwe kabili iyafudumeza, iyihlukanisa ibe yimicu emibili eyodwa. Izinga lokushisa liyehliswa ngesikhathi sesigaba sokungenisa izikhalazo ukuvumela ama-premers ukuthi ahlanganise ukulandelana kwe-DNA okuhlosiwe. Ekugcineni, amazinga okushisa akhuphuka futhi ukungena esigabeni esisezingeni eliphezulu, lapho i-DNA polytherase syntherizes i-New DNA strands.

Izici eziphambili ze-Thermal Cypeer

Ama-cyplers anamuhla afudumele ahlome ngezinto ezahlukahlukene ezithuthukisa ukusebenza kwazo nokusebenziseka kwazo. Enye yentuthuko ebaluleke kakhulu yikhono lokuhlela imijikelezo yokushisa eminingi, evumela abaphenyi ukuthi benze ngezifiso amaphrotholi abo e-PCR. Abakhi be-Thermal abaningi bafaka nama-lids afudumele avimbela ukubuyiselwa emuva ekwakheni amashubhu okusabela, aqinisekise izimo ezifanele zokwenziwa.
Enye into ephawuleka ukuhlanganiswa kokusebenza kwe-PCR yesikhathi sangempela. Ama-cyplers wesikhathi sangempela anika amandla abaphenyi ukuthi baqaphe inqubo ye-Amplification ngesikhathi sangempela, bahlinzeka ngedatha yenani ngenani le-DNA elikhiqizwayo. Lesi sici siwusizo ikakhulukazi kuzinhlelo zokusebenza ezifana ne-PCR (QPCR), lapho izilinganiso eziqondile zibalulekile ekutholeni imiphumela efanele.

Ukusetshenziswa kwe-Thermal Cypeer

Ukusetshenziswa kwamabhayisekili afudumele kubanzi futhi kuyahlukahluka. Ekuhlolweni kwemitholampilo, basetshenziselwa ukuthola amagciwane, ukuguqulwa kwezakhi zofuzo, kanye nezifo ezizuze njengefa. Isibonelo, ngesikhathi sobhubhane lwe-Covil-19, amakhiloshi ashisayo adlale indima ebalulekile kumasampula wokuhlola ngokushesha, asiza ekuboneni abantu abanegciwane futhi balawule ukusakazeka kwaleli gciwane.
Ezitolo ezicwaningayo, abashayeli bamabhayisekili afudumele babucayi ngoGene Cloning, Ukuhlelwa kabusha, kanye nezifundo zezincazelo zofuzo. Bavumela ososayensi ukuthi bahlole ukuhlukahluka kofuzo futhi baqonde izindlela ezingaphansi kwezifo. Ngaphezu kwalokho, kwezeluleko ze-agvign zezolimo, amakhilogremu ashisayo asetshenziselwa ukuthuthukisa izidalwa eziguqulwe ngofuzo (GMOs) ezingamelana nengcindezi yemvelo noma zithuthukise okuqukethwe okunempilo.

Ikusasa Lama-Thermal Cyplers

Njengoba ubuchwepheshe buqhubeka buvela, ngakho-ke yenza amakhilogremu ashisayo. Ukusungulwa okufana nokuncane nokuhlanganiswa namapulatifomu edijithali asezingeni eliphakeme. Le nqubekela phambili kulindeleke ukuthi yenze ama-cyplers afudumele afinyeleleke kakhudlwana futhi asebenziseka kalula, avumele abaphenyi ukuthi benze izivivinyo ngokusebenza okukhulu nokunemba.
Ngokwengeziwe, ukukhuphuka kwe-biology yokwenziwa kanye nomuthi owenziwe ngezifiso kungashayela okunye ukuthuthukiswa kobuchwepheshe be-cycleer technology. Njengoba abacwaningi bafuna ukukhohlisa ngokunembile okokusebenzisa izakhi zofuzo, isidingo sabagibeli bamakhilogremu athuthukile abakwazi ukuvumelanisa izivumelwano eziyinkimbinkimbi zizokwanda kuphela.

Ekuphetheni

Le khasiI-Thermal Cypeer kungaphezu nje kwensiza yelabhoratri; Kuyisango lokuqonda ubulukhuni bempilo ezingeni lamangqamuzana. Ikhono layo lokukhulisa i-DNA seliguqule izinkambu elungiselelweni kwezolimo, okwenza kube yithuluzi elibalulekile ekuphishekeleni ulwazi nokuvuselelwa. Ukubheka ikusasa, ngokungangabazeki amakhilomitha ashisayo azoqhubeka nokudlala indima ebalulekile ekubumbeni insimu ye-biotechnology kanye nocwaningo lwe-molecular.