Ukuvula Izimfihlo Zempilo: Ukubaluleka kwama-nucleic acid aldaction kits

Emkhakheni we-biology we-molecular, ukukhishwa kwama-nucleic acid (DNA ne-RNA) yisinyathelo esiyisisekelo, siveza indlela yezinhlelo zokusebenza ezingenakubalwa ezivela ekuhlolweni kwezakhi zofuzo. I-Nucleic Acid Extraction Kits iguqule le nqubo, okwenza isebenze kahle, ithembekile, futhi itholakale kubacwaningi kanye nama-laboratories emhlabeni jikelele. Kule bhulogi, sizohlola la makhithi, ukubaluleka kwezingxenye zabo, kanye nomthelela wazo ekuthuthukisweni kwesayensi.

Yini ikhithi ye-nucleic acid kit?

I-Nucleic Acid Extraction KitsAmathuluzi aklanyelwe ukuhlukanisa i-DNA noma i-RNA kusuka ezinhlobonhlobo zamasampula aphilayo, afana negazi, izicubu, amaseli, kanye namasampula wezemvelo. Lawa ma-kits ngokuvamile aqukethe wonke ama-reagents namaphrothokholi adingekayo ukwenza lula inqubo yokukhishwa, eqinisekisa abacwaningi abangathola ama-nucleic acid aphezulu anokungcola okuncane.

Inqubo yokukhishwa

Inqubo yokukhishwa imvamisa ifaka izinyathelo ezimbalwa ezibalulekile: I-Celly Gysis, Ukuhlanzwa, kanye nokuqedwa.

I-Cell lysis: Isinyathelo sokuqala ukuvula amaseli ukukhulula ama-nucleic acid. Lokhu kuvame ukufezekiswa kusetshenziswa i-lysis buffer equkethe okokuhlanza kanye nama-enzyme aphazamisa ama-membrane weseli kanye namaprotheni edevu.

Ukuhlanzwa: Ngemuva kokukhishwa kwama-acid ama-nucleic acid, isinyathelo esilandelayo ukususa amaprotheni anjengamaprotheni, ama-lipid, nezinye izichitho zeselula. Ama-kits amaningi asebenzisa amakholomu e-silica noma ubuhlalu bakazibuthe bokukhetha ama-nucleic acid, ngaleyo ndlela ugeza ukungcola.

Ukuqedwa: Ekugcineni, ama-nucleic acid ahlanzekile afakwa ku-buffer efanelekile, alungele izinhlelo zokusebenza ezaphansi ezifana ne-PCR, ukulandelana, noma ukugcwala.

Kungani usebenzise i-nucleic acid extraction kit?

Ukusebenza kahle: Izindlela zokukhishwa kwendabuko ze-nucleic acid zidla isikhathi futhi zisebenza kanzima. I-nucleic acid extraction Kits yenza lula inqubo futhi ngokuvamile ingaqedela ukukhishwa kungakapheli ihora.

Ukungaguquguquki: amaphrothokholi ajwayelekile anikezwe yilezi zikhithi aqinisekise ukuvela kabusha kanye nokwethenjwa kwemiphumela. Lokhu kubalulekile ekuhlolweni lapho ukunemba kubalulekile, njengokuxilongwa kwemitholampilo noma ucwaningo.

Ukuguquguquka: Ama-Kits amaningi enzelwe ukuphatha izinhlobo ezahlukene zesampula, azibekele zilungele izinhlelo zokusebenza ezahlukahlukene. Noma ngabe usebenza namasampula abantu, izicubu zezitshalo, noma amasiko ama-microbial, kungenzeka ukuthi ikhithi ukuze ivumelane nezidingo zakho.

I-SERNECY EBUCHWEPHESHE: Iningi le-nucleic acid extraction kits iza nemiyalo enemininingwane futhi zenzelwe ukuba kube lula ukuyisebenzisa, ngisho nakulabo okungenzeka bangabi nakho okuhlangenwe nakho okubanzi kwelebhu. Lokhu kufinyelela ngentando yeningi kumasu we-molecular biology, okuvumela abacwaningi abaningi ukuthi babambe iqhaza ocwaningweni lwezakhi zofuzo.

Ukusetshenziswa kwe-nucleic acid extraction

Ama-acid ama-nucleic atholwe kulawa makhithi angasebenza njengesisekelo sezicelo eziningi:

Ucwaningo lweGene: Qonda umsebenzi wofuzo, ukuvezwa kanye nomthethonqubo.
Ukuxilongwa kwemitholampilo: Ukutholwa kwezifo zofuzo, izifo ezithathelwanayo kanye nomdlavuza.
Isayensi ye-Forensic: Ukuhlaziywa kwamasampula we-DNA ophenya lobugebengu.
I-Biotechnology Ezolimo: Ukuthuthukiswa kwezinto eziguqulwe ngofuzo (GMOs) ukukhulisa izivuno zezilimo.
Ekuphetheni
I-Nucleic Acid Extraction Kitsbangamathuluzi asemqoka e-biology yesimanjemanje ye-molecular, evumela abaphenyi ukuthi bavule izimfihlo zokuphila ezingeni lamangqamuzana. Ukusebenza kwazo, ukuvumelana, nokuguquguquka kwezinto eziguquguqukayo kushintshe isimo socwaningo lwezakhi zofuzo nokuxilonga, okwenza kube lula kunanini ngaphambili ukuhlola ubulukhuni be-DNA ne-RNA. Njengoba ubuchwepheshe buqhubeka buqhubekisela phambili, singalindela ukuthi lezi zikhithi ziphenduke futhi, zivule iminyango emisha ekutholweni kwesayensi nasekuqanjwe okusha. Noma ngabe ungumcwaningi onolwazi noma okusha ensimini, ukutshala imali kwi-Nucleic Acid Extraction Kit kungathuthukisa kakhulu ikhwalithi yomsebenzi wakho futhi ufake isandla emzimbeni owandise ulwazi ngohlobo lwezakhi zofuzo.