Ukuhlukahluka Kwezicwecwe Ezijulile Ocwaningweni Lwelabhorethri

Amapuleti omthombo ojulileziyisisekelo ocwaningweni lwaselabhorethri, zihlinzeka ngezixazululo eziguquguqukayo nezisebenzayo zezinhlelo zokusebenza ezahlukahlukene. Lawa mapuleti e-multiwell aklanyelwe ukuthwala amasampula ngendlela ephumelela kakhulu, okuwenza abe yithuluzi elibalulekile emikhakheni eyahlukene yesayensi efana ne-genomics, i-proteomics, ukutholwa kwezidakamizwa, nokunye.

Enye yezinzuzo eziyinhloko zamapuleti omthombo ojulile yikhono lawo lokuphatha amasampula amakhulu. Lawa mapuleti anokujula kwemithombo okusuka ku-2 kuye ku-5 mm futhi angakwazi ukufaka amasampula amavolumu afika ku-2 ml emthonjeni ngamunye, okuwenza alungele izinhlelo zokusebenza ezidinga ukucutshungulwa kwamavolumu amakhulu amasampula. Lesi sici sibaluleke kakhulu ekuhlolweni kokuhlolwa komphumela omkhulu lapho amasampuli amaningi adinga ukucutshungulwa kanyekanye.

Ngokungeziwe kumthamo wesampula ephezulu, amapuleti emithombo ejulile ahambisana nezinhlobonhlobo zezinsimbi zaselabhorethri, okuhlanganisa amasistimu okuphatha uketshezi oluzenzakalelayo, ama-centrifuges, nezifundi zamapuleti. Lokhu kuhambisana kuvumela ukuhlanganiswa okungenamthungo ekugelezeni komsebenzi kwaselabhorethri okukhona, izinqubo ezilula nokukhulisa ukusebenza kahle. Ukuthi asetshenziselwa ukulungiselela isampula, ukugcinwa noma ukuhlaziya, amapuleti emithombo ejulile ahlinzeka ngenkundla enokwethenjelwa nefanelekile yokwenza izivivinyo.

Ukwengeza, amapuleti ajulile atholakala ngezindlela ezihlukahlukene, kufaka phakathi ukulungiswa kwe-96-, 384-, kanye ne-1536-well, ukunikeza abacwaningi ukuguquguquka okusekelwe ezidingweni zabo zokuhlola ezithile. Lokhu kuvumelana nezimo kwenza amapuleti ajulile afanele ukusetshenziswa okuhlukahlukene, kusukela kusiko lamaseli kanye nokukhula kwe-microbial kuya ku-protein crystallization kanye nokuhlolwa okuhlanganisiwe.

Idizayini yamapuleti emithombo ejulile iwenza alungele ukugcinwa nokugcinwa kwesampula. Ukwakhiwa kwazo okuqinile nokuhambisana nezinketho zokuvala ezifana namafilimu anamathelayo nama-lid gaskets aqinisekisa ubuqotho besampula futhi anciphise ingozi yokungcola. Lokhu kwenza amapuleti emithombo ejulile alungele ukugcinwa isikhathi eside kwamasampula ezinto eziphilayo, ama-reagents nezinhlanganisela, okuhlinzeka abacwaningi ngesixazululo esinokwethenjelwa sokuphatha isampula.

Ukwengeza, amapuleti omthombo ojulile atholakala ezintweni ezihlukahlukene, kufaka phakathi i-polypropylene ne-polystyrene, ngayinye enezinzuzo eziyingqayizivele kuye ngokuthi isicelo. Isibonelo, amapuleti e-polypropylene ajulile ayaziwa ngokumelana kwawo namakhemikhali nokuhambisana nezinhlobonhlobo eziningi ze-solvents, okuwenza afanelekele ukusetshenziswa okubandakanya amakhemikhali anokhahlo. Ngakolunye uhlangothi, amapuleti e-polystyrene deep-well, avame ukuthandwa ngenxa yokucaca kwawo kokubona, okuwenza alungele izinhlelo zokusebenza ezidinga ukuhlolwa okubonakalayo noma ukutholwa kwe-fluorescence.

Ngokufigqiwe,amapuleti omthombo ojulileayithuluzi elibalulekile ocwaningweni lwaselabhorethri, ehlinzeka ngokuguquguquka, ukusebenza kahle, nokuthembeka ezinhlobonhlobo zezinhlelo zokusebenza. Umthamo wabo wesampula ophezulu, ukuhambisana nezinsimbi zaselabhorethri, kanye nokuguquguquka kwamafomethi nezinto zokwakha kuzenza zibe izimpahla ezibalulekile kubacwaningi emikhakheni eyahlukene yesayensi. Kungakhathaliseki ukuthi okokucubungula amasampula, ukugcinwa noma ukuhlaziya, amapuleti emithombo ejulile ayaqhubeka nokubamba iqhaza elibalulekile ekuthuthukiseni ukutholwa kwesayensi nokusungula izinto ezintsha.