Ukuguquguquka Kwamapuleti Omthombo Ojulile Ocwaningweni Lwelabhorethri

Amapuleti omthombo ojulileziyisisekelo ocwaningweni lwelabhorethri, zinikeza izixazululo eziguquguqukayo nezisebenzayo zezinhlelo zokusebenza ezahlukahlukene. Lawa ma-multiwell plate aklanyelwe ukwamukela amasampula ngendlela ephumelela kakhulu, okwenza abe ithuluzi elibalulekile emikhakheni eyahlukene yesayensi efana ne-genomics, i-proteomics, ukutholakala kwezidakamizwa, nokuningi.

Enye yezinzuzo eziyinhloko zamapuleti omthombo ojulile yikhono lawo lokusingatha inani elikhulu lamasampula. Lawa mapuleti anokujula kwemithombo okusukela ku-2 kuya ku-5 mm futhi angamukela inani lamasampula afinyelela ku-2 ml ngomthombo ngamunye, okwenza abe mahle kakhulu ekusetshenzisweni okudinga ukucutshungulwa kwenani elikhulu lamasampula. Lesi sici sibaluleke kakhulu ekuhlolweni kokuhlolwa okuphezulu lapho amasampula amaningi kudingeka acutshungulwe ngasikhathi sinye.

Ngaphezu komthamo omkhulu wamasampula, amapuleti emithombo ejulile ayahambisana nezinsimbi ezahlukahlukene zelebhu, okuhlanganisa izinhlelo zokuphatha uketshezi ezenzakalelayo, ama-centrifuge, kanye nezifundi zamapuleti. Lokhu kuhambisana kuvumela ukuhlanganiswa okungenamthungo emisebenzini yelebhu ekhona, ukwenza lula izinqubo kanye nokwandisa ukusebenza kahle. Kungakhathaliseki ukuthi kusetshenziselwa ukulungiselela amasampula, ukugcina noma ukuhlaziya, amapuleti emithombo ejulile ahlinzeka ngeplatifomu ethembekile nelula yokwenza izivivinyo.

Ngaphezu kwalokho, amapuleti e-deep-well atholakala ngezindlela ezahlukahlukene, kufaka phakathi ukwakheka kwe-96-, 384-, kanye ne-1536-well, okunikeza abacwaningi ukuguquguquka okusekelwe ezidingweni zabo ezithile zokuhlola. Lokhu kuvumelana nezimo kwenza amapuleti e-deep-well afanelekele izinhlelo zokusebenza ezahlukahlukene, kusukela ekukhuleni kwamaseli kanye nokukhula kwamagciwane kuya ekubunjweni kwama-protein kanye nokuhlolwa okuhlanganisiwe.

Ukwakheka kwamapuleti omthombo ojulile kuwenza abe mahle kakhulu ekugcineni nasekulondolozweni kwamasampula. Ukwakhiwa kwawo okuqinile kanye nokuhambisana nezinketho zokuvala ezifana namafilimu okunamathelayo kanye nama-gasket esivalo kuqinisekisa ubuqotho besampula futhi kunciphisa ingozi yokungcola. Lokhu kwenza amapuleti omthombo ojulile abe mahle kakhulu ekugcineni isikhathi eside amasampula ezinto eziphilayo, ama-reagent kanye nama-compound, okunikeza abacwaningi ikhambi lokuphatha amasampula elithembekile.

Ngaphezu kwalokho, amapuleti emithombo ejulile atholakala ngezinto ezahlukahlukene, kufaka phakathi i-polypropylene ne-polystyrene, ngalinye linezinzuzo ezihlukile kuye ngokusetshenziswa. Isibonelo, amapuleti emithombo ejulile ye-polypropylene aziwa ngokumelana kwawo namakhemikhali kanye nokuhambisana nezinhlobo eziningi zezinyibilikisi, okwenza afaneleke ukusetshenziswa okubandakanya amakhemikhali anamandla. Amapuleti emithombo ejulile ye-polystyrene, ngakolunye uhlangothi, avame ukuthandwa ngenxa yokucaca kwawo kokukhanya, okwenza afaneleke kakhulu ukusetshenziswa okudinga ukuhlolwa okubonakalayo noma ukutholwa kwe-fluorescence.

Ngokufigqiwe,amapuleti omthombo ojulileziyithuluzi elibalulekile ocwaningweni lwelabhorethri, ezihlinzeka ngokuguquguquka, ukusebenza kahle, kanye nokuthembeka ezinhlobonhlobo zezicelo. Amandla azo aphezulu esampula, ukuhambisana nezinsimbi zelebhu, kanye nokuguquguquka kwefomethi nezinto zokwakha kuzenza zibe yimpahla ebalulekile kubacwaningi emikhakheni eyahlukene yesayensi. Kungakhathaliseki ukuthi zicutshungulwa, zigcinwe noma zihlaziywe, amapuleti emithombo ejulile aqhubeka nokudlala indima ebalulekile ekuthuthukiseni ukutholakala kwesayensi kanye nokusungula izinto ezintsha.