Yiziphi Izinkinga Ezivamile Ngama-Thermocycler?

 

Izinkinga Zokulingana Kwezinga Lokushisa

Inkinga ebaluleke kakhulu ye-thermocycler ihilela ukungalingani kwezinga lokushisa kulo lonke ibhloko. Ukushisa okungalingani kudala imiphumela yokukhulisa okuguquguqukayo, okubeka engcupheni ukuthembeka kokuhlolwa.I-FastCycler Thermal CyclerAmamodeli asebenzisa izakhi zePeltier ezithuthukisiwe kanye nama-algorithms ayinkimbinkimbi ukuze alondoloze ukufana okungu-±0.2°C kuyo yonke imithombo. Kodwa-ke, amabhlogo okushisa agugayo, i-thermal paste egugile, noma imfucumfucu eqongelelekile ingonakalisa ukusebenza ngokuhamba kwesikhathi.

Izimpawu zezinkinga zokushisa: Ukusabela kwe-PCR okuhlulekile ezindaweni ezithile zomthombo, ama-curve okuncibilika angaguquki, noma isivuno somkhiqizo esiguquguqukayo kupuleti elifanayo lesampula kubonisa izinkinga ezingaba khona zokufana ezidinga ukulinganiswa okusheshayo.

Ukungasebenzi kahle kokushisa kwesivalo

Izivalo ezifudumele zivimbela ukuminyana okunciphisa ukuxubana kokusabela futhi kunciphisa ukusebenza kahle kwe-PCR. Ukwehluleka kokushisa kwesivalo kuphakathi kwezikhalazo ezivame kakhulu ze-thermocycler. Ukushisa okunganele kwesivalo kuvumela ukwakheka kokuminyana, kuyilapho ukushisa ngokweqile kungalimaza amasampula noma kulimaze izinto ezisetshenziswayo zepulasitiki.

Izinhlelo zesimanje ze-FastCycler Thermal Cycler zinokufudumeza kwesivalo okulungisekayo (ngokuvamile okungu-100-110°C) ngokulawula izinga lokushisa okunembile. Ukulungiswa njalo kuhlanganisa ukuhlola izindlela zokucindezela kwesivalo kanye nezinzwa zokushisa ukuqinisekisa ukuthintana okufanele kanye nokusatshalaliswa kokushisa.

Ukuwohloka Kwezinga Lokukhuphuka

Ijubane lokusheshisa okusheshayo lihlukanisa ama-thermocycler e-premium kumamodeli ayisisekelo. Ngokuhamba kwesikhathi, amazinga okushisa nokupholisa angase ehle ngenxa yokuguguleka kwe-Peltier element, ukwehluleka kwefeni, noma izinkinga zesiqandisi ezinhlelweni zokupholisa. Lokhu konakala kwandisa izikhathi zomjikelezo futhi kungathinta ukusabela okuzwela ukushisa.

Imishini ye-FastCycler Thermal Cycler yezinga lobuchwepheshe igcina ukusheshisa okusheshayo (4-5°C/sekondi) ngokusebenzisa ama-Peltier aphindwe kabili kanye nokuphathwa kokushisa okulungiselelwe. Uma uthenga, qinisekisa kokubili imininingwane yokushisa kanye nokupholisa, hhayi nje amazinga aphezulu okusheshisa.

Izinkinga zeSoftware kanye nokuxhumana

Ama-thermocycler anamuhla ahlanganisa isofthiwe eyinkimbinkimbi yokuhlela iphrothokholi, ukuloga idatha, kanye nokuxhumeka kwenethiwekhi. Izinkinga ezivamile zesofthiwe zifaka:

Amaphutha e-firmware: Kubangela ukuphahlazeka kohlelo noma ukufundwa kwezinga lokushisa okungalungile

Ukwehluleka kwe-USB/Ethernet: Ukuvimbela ukudluliswa kwedatha noma ukuqapha okude

Ukungasebenzi kahle kwesikrini sokuthinta: Ukwenza kube nzima ukuhlela iphrothokholi

Izinkinga zokuhambisana: Ngezinhlelo zokuphathwa kolwazi lwelabhorethri (i-LIMS)

Abakhiqizi abaphambili banikeza izibuyekezo ze-firmware njalo kanye nokusekelwa kobuchwepheshe okusabelayo ukuze kuxazululwe lezi zinselele ngokushesha.

Ukuguga Nokudabuka Kwemishini

Izingxenye zomzimba ziwohloka kancane kancane:

Ukungcola kwamabhulokhiAmasampula achithekile adala ukuthintana okungalingani kokushisa okudinga ukuhlanzwa kahle

Umonakalo we-hinge yesivalo: Ukuvulwa njalo kwenza izingxenye zemishini zibe buthaka

Ukwehluleka kwabalandeli: Ukwehlisa ukusebenza kahle kokupholisa kanye nokwandisa izikhathi zomjikelezo

Ukuzulazula kwenzwa: Kubangela ukufundwa kwezinga lokushisa okungafanele okudinga ukulungiswa kabusha

Ukushintshashintsha Kokulinganisa

Zonke izinjini zokushisa zidinga ukuqinisekiswa kokulinganisa njalo. Izinzwa zokushisa ziyashintsha ngokwemvelo ngokuhamba kwesikhathi, okungase kubangele amaphutha amakhulu okuhlola. Amalabhorethri ochwepheshe kufanele enze ukuhlolwa kokulinganisa kwekota ngayinye esebenzisa ama-thermometer okubhekisela aqinisekisiwe.

IkhwalithiI-FastCycler Thermal CyclerAmamodeli afaka izici zokuzihlola ezixwayisa abasebenzisi ngezidingo zokulinganisa ngaphambi kokuba izinkinga zithinte imiphumela. Ezinye izinhlelo ezithuthukisiwe zinikeza izinqubo zokulinganisa okuzenzakalelayo ezinciphisa ukungenelela ngesandla.

Amasu Okulungisa Okuvimbelayo

Nciphisa izinkinga ze-thermocycler ngokulungisa ngokushesha:

• Hlanza amabhlogo okushisa njalo ngenyanga usebenzisa izinyibilikisi ezifanele
• Qinisekisa ukunemba kwezinga lokushisa njalo ngekota ngama-probe alinganisiwe
• Buyekeza i-firmware njalo ukuze ufinyelele ukuthuthukiswa nokulungiswa kwamaphutha
• Shintsha izinto ezisetshenziswayo (ama-gasket esivalo, ama-thermal pad) ngokwesheduli yomkhiqizi
• Gcina umoya ungena kahle eduze kwemishini ukuze uphole kahle

Ukukhetha Imishini Ethembekile

Uma uthenga ama-thermocycler, beka phambili abakhiqizi abanikeza:

Iziqinisekiso eziphelele: Ukumboza izingxenye kanye nomsebenzi

Usekelo lobuchwepheshe olusabelayo: Ngokutholakala okusheshayo kwezingxenye ezithathelwanayo

Irekhodi eliqinisekisiwe: Kuboniswe ukwethembeka kuma-laboratory ontanga

Ukulungiswa okulula ukukusebenzisa: Izingxenye ezifinyelelekayo kanye nemibhalo yesevisi ecacile

Isiphetho

Nakuba ama-thermocycler engaletha izinselele ezahlukene zokusebenza, ukuqonda izinkinga ezivamile kunika amandla ukukhethwa kwemishini enolwazi kanye nokuhlela ukulungiswa okufanele. Ukutshala imali ezinhlelweni ze-FastCycler Thermal Cycler ezisezingeni eliphezulu ezinengqalasizinda yokusekela eqinile kunciphisa isikhathi sokungasebenzi futhi kuqinisekisa imiphumela ye-PCR eqhubekayo nethembekile. Hlola izindleko eziphelele zobunikazi—kufaka phakathi izidingo zokulungisa kanye nekhwalithi yokusekela—kunentengo yokuthenga yodwa. I-thermocycler efanele iba umlingani wezelebhu othembekile oletha iminyaka yokusebenza okungenazinkinga kanye nemiphumela yesayensi ephindaphindwayo.