Uhlelo lokuhlanza i-nucleic acid

Isistimu yokuhlanza i-nucleic acid efakiwe isithombe

Incazelo emfushane:

Usebenzisa ubuchwepheshe be-magnetic bead desation technology, khetha ikhithi efanelekile ingahlukanisa ngokuzenzakalelayo futhi ihlanze i-nucleic acid ephezulu ye-nucleic kusuka ezintweni ezahlukahlukene (igazi, izicubu, iseli). Ithuluzi linomklamo wesakhiwo esihle, imisebenzi ephelele yokugqilazwa kwe-ultraviolet kanye nokushisa, kanye nesikrini esikhulu sokuthinta kulula ukusebenza. Kungumsizi ophumelelayo wokutholwa kwamangqamuzana emtholampilo kanye ne-molecular biology elebhu yesayensi.

XHUMANA NATHI Ibhukwana Lomkhiqizo

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Izici zomkhiqizo

I-1, uhlelo lokulawula lwezimboni lwenza izimboni lwenza ukusebenza okuzinzile kwamahora angama-24
2, isivuno somkhiqizo ophakeme kanye nobumsulwa obuhle
I-3, ukucubungula okuzenzakalelayo kwe-nucleic acid expection kungenziwa kumasampula angama-32/96 ngasikhathi sinye, kukhulula kakhulu izandla zabaphenyi
I-4, ama-realegents asekelayo angasetshenziswa kumasampula ahlukahlukene anjengeSwabs, i-serum plasma, izicubu, izitshalo, igazi lonke, futhi zinokucaciswa okuningi kwe-single / 16t / 32t / 48t
Ukuhlangabezana nezidingo zamakhasimende ezahlukene
I-5, i-self actillent acpecial software kanye nesikrini sokuthinta yenza ukusebenza kube lula futhi kusheshe
I-6, Sheath elahlekile ifaka izinduku kazibuthe kanye namasampula, futhi umshini uhlome nge-UV inzalo ye-UV kanye nezinhlelo zomoya we-podtration adsorption ukwenqaba ukungcoliswa kwesiphambano

(Imiphumela yokuhlola)

Imiphumela yokuhlolwa kwe-electrophoresis ye-Faeces

namasampula enhlabathi ngemuva kokukhishwa

(Imiphumela yokuhlola)

Isampula ye-UU ekhishwe imiphumela yokuhlaziya ye-QPCR

(Kufaka phakathi okujwayelekile kwangaphakathi)

(Imiphumela yokuhlola)

I-NG isampula ekhishwe imiphumela yokuhlaziya ye-QPCR

(Kufaka phakathi okujwayelekile kwangaphakathi)

Cha.	ukuthayipha	inkuthazo	umuvo	A260	A280	260/280	260/230	imbonakaliso
1	Uhlobo lwanamvubelo	556.505	μg / ml	13.913	I-6.636	I-2.097	2.393	ubende
2	Uhlobo lwanamvubelo	540.713	μg / ml	13.518	6.441	I-2.099	I-2.079	ubende
3	Uhlobo lwanamvubelo	799.469	μg / ml	19.987	9.558	I-2.091	2.352	insonge yasolwandle
4	Uhlobo lwanamvubelo	847.294	μg / ml	21.182	10.133	I-2.090	2.269	insonge yasolwandle
5	Uhlobo lwanamvubelo	I-1087.187	μg / ml	27.180	12.870	2.112	2.344	isibindi
6	Uhlobo lwanamvubelo	980.632	μg / ml	24.516	11.626	2.109	2.329	isibindi