Ikhithi Yokuthola I-SARS-CoV-2 Nucleic Acid (i-Fluorescence RT-PCR)

Incazelo emfushane:

Ikhithi yokuthola i-nucleic acid ye-SARS-COV-2 (i-Fluorescence RT-PCR) ingasetshenziswa ukuthola i-nucleic acid ye-coronavirus entsha, esetshenziselwa ukuxilongwa okusizayo kanye nokuhlolwa kwe-epid-emiological kokutheleleka okusha kwe-coronavirus, efanelekile kwi-CDC, ezibhedlela, elabhorethri yezokwelapha evela eceleni, esikhungweni sokuhlolwa ngokomzimba kanye nakwamanye amalabhorethri emitholampilo.

XHUMANA NATHI

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Izici Zomkhiqizo

1, Ukuzwela Okuphezulu: Umkhawulo Wokuthola (LoD)＜Amakhophi angu-2×102/ml.

2, I-gene eqondiwe kabili: Thola i-gene ye-ORFlab kanye ne-gene ye-N ngesikhathi esisodwa, landela umthetho we-WHO.

3, Ifanelekela amadivayisi ahlukahlukene: ABI 7500/7500FAST; Roche LightCycler480; BioRad CFX96; I-BigFish-BFQP96/48 yethu.

4, Okusheshayo nokulula: I-reagent exutshwe kusengaphambili kulula ukuyisebenzisa, amakhasimende adinga nje ukwengeza i-enzyme kanye nethempulethi. Ikhithi yokukhipha i-nucleic acid ye-Bigfish ifanelana kahle nalolu vivinyo. Ngokusebenzisa umshini wokukhipha ozenzakalelayo ogcwele, kuyashesha ukucubungula amasampula amaningi.

5, Ukuphepha kwe-Bio: I-Bigfish inikeza i-Sample Preservative Liquid ukuze ivimbele ngokushesha igciwane ukuqinisekisa ukuphepha kwabasebenzi.