Isibambi Sekhithi Yokuhlola Eyodwa Ye-Nucleic Acid Isizinda

Isingeniso Somkhiqizo

Ikhithi yokukhipha i-nucleic acid ye-MagPure ihlinzeka ngendlela elula kakhulu, esheshayo futhi engabizi kakhulu yokuhlukaniswa kwe-DNA noma i-RNA kwekhwalithi ephezulu ngokusekelwe endleleni yobuhlalu kazibuthe. Ikhithi yokukhipha i-nucleic acid ye-MagPure ayiqukethe i-solvent eyingozi futhi ifaneleka kahle ekucubungulweni kwamasampuli ahlukahlukene. Lobu buchwepheshe bobunikazi buqeda isidingo se-centrifugation, i-vacuum filtration noma ukuhlukaniswa kwekholomu, ngaleyo ndlela kwandise ukuphuma kwesampula nokuthuthukisa ukuphindaphinda. I-DNA noma i-RNA ehlanzwe yi-MagPure isilungele ukusetshenziswa kuzo zonke izinhlobo zezinhlelo zokusebenza zebhayoloji yamangqamuzana njenge-PCR, ukulandelana, izinqubo zokucisha, ukuhlaziywa kwe-mutant kanye ne-SNP. I-MagPure nucleic acid extraction kit ifanele ukusetshenziswa ngegazi elivame ukuphathwa ngama-anticoagulants afana ne-citrate, i-heparin noma i-EDTA, uketshezi lwebhayoloji, izicubu ezifakwe uParafini, izicubu zezilwane noma zezitshalo, amaseli akhulisiwe, amaseli ebhaktheriya aphethe isampula ye-plasmid negciwane. Ikhithi yokukhipha i-MagPure nucleic acid isetshenziswa ngokulungiswa okujwayelekile okujwayelekile kwesampula-isampula, ukubopha kazibuthe, ukuwashwa kanye nokuluthwa. Futhi ngokusekela ukusetshenziswa kwezinsimbi zokuhlanza ze-BigFish NUETRACTION, amakhasimende athola ukukhishwa okusheshayo nokuphezulu kwe-DNA noma i-RNA.