Isibambi Sekhithi Yokuhlola Eyodwa Yokukhipha I-Nucleic Acid

Isingeniso Somkhiqizo

Ikhithi yokukhipha i-nucleic acid ye-MagPure inikeza indlela elula kakhulu, esheshayo futhi engabizi kakhulu yokuhlukanisa i-DNA noma i-RNA ngekhwalithi ephezulu ngokusekelwe endleleni yobuhlalu be-magnetic. Ikhithi yokukhipha i-nucleic acid ye-MagPure ayinayo i-solvent eyingozi yemvelo futhi ifaneleka kahle ekucutshungulweni kwamasampula ahlukahlukene. Lobu buchwepheshe obukhethekile buqeda isidingo se-centrifugation, i-vacuum filtration noma ukuhlukaniswa kwekholomu, ngaleyo ndlela kwandisa ukuphuma kwesampula futhi kuthuthukiswe ukuzala kabusha. I-DNA noma i-RNA ehlanzwa yi-MagPure ilungele ukusetshenziswa kuzo zonke izinhlobo zezicelo ze-molecular biology njenge-PCR, ukulandelana, izinqubo zokususa amabala, ukuhlaziywa kwe-mutant kanye ne-SNP. Ikhithi yokukhipha i-nucleic acid ye-MagPure ifanele ukusetshenziswa negazi elivame ukwelashwa ngama-anticoagulants njenge-citrate, i-heparin noma i-EDTA, uketshezi lwezinto eziphilayo, izicubu ezifakwe i-paraffin, izicubu zezilwane noma zezitshalo, amaseli akhuliswe, amaseli ebhaktheriya athwala isampula ye-plasmid kanye negciwane. Ikhithi yokukhipha i-nucleic acid ye-MagPure isetshenziswa ngokulungiselela isampula ejwayelekile eyodwa elula, ukubopha nge-magnetic, ukugeza kanye nokususa. Futhi ngokusekela ukusetshenziswa kwamathuluzi okuhlanza e-BigFish NUETRACTION, amakhasimende afinyelela ukukhishwa kwe-DNA noma i-RNA okusheshayo nokuphezulu.