Ukukhishwa kwe-DNA/RNA

Isingeniso somkhiqizo:

Kusetshenziswa ubuchwepheshe bokuhlanza ubuhlalu obunobuthi, ikhithi yokuhlanza i-DNA/RNA yegciwane le-Magpure ingakhipha i-DNA/RNA yamagciwane ahlukahlukene njengegciwane le-African Swine Fever kanye ne-coronavirus entsha kumasampula ahlukahlukene njenge-serum, i-plasma kanye nesisombululo sokucwiliswa kwe-swab, futhi ingasetshenziswa ekuhlolweni kwe-PCR/RT-PCR okuphansi, ukulandelana, ukuhlaziywa kwe-polymorphism kanye nokunye ukuhlolwa kokuhlaziywa kwe-nucleic acid kanye nokutholwa. Ifakwe ithuluzi lokuhlanza i-nucleic acid elizenzakalelayo ngokuphelele kanye nekhithi yokulayisha kusengaphambili, ingaqeda ngokushesha ukukhipha inani elikhulu lamasampula e-nucleic acid.

Izici Zomkhiqizo:

1. Kuphephile ukuyisebenzisa, ngaphandle kwe-reagent enobuthi
2. Kulula ukuyisebenzisa, asikho isidingo se-Proteinase K kanye ne-Carrier RNA
3. Khipha i-DNA/RNA yegciwane ngokushesha nangendlela ephumelelayo ngokuzwela okuphezulu
4. Ukuthutha nokugcina endaweni yokushisa yegumbi.
5. Ifanelekela ukuhlanzwa kwe-nucleic acid okuhlukahlukene kwegciwane
6. Ifakwe ithuluzi lokuhlanza i-nucleic acid elizenzakalelayo ngokuphelele ukuze icubungule amasampula angu-32 zingakapheli imizuzu engama-30.