Ikhithi Yokuhlanza I-DNA Yegazi Le-MagaPure

Incazelo emfushane:

Le khithi iqukethe ama-microspheres e-superparamagnetic kanye ne-buffer yokukhipha eyenziwe ngaphambilini, futhi ifaneleka ekukhishweni okulula nokuphumelelayo kwe-DNA ye-genomic kusuka kumasampula egazi aphelele amasha, aqandisiwe, futhi agcinwe isikhathi eside. Izingcezu ze-DNA ye-genomic ezikhishwe zinkulu, zimsulwa kakhulu, futhi zisezingeni elizinzile nelithembekile. I-DNA ekhishwe ifanelekela izivivinyo ezahlukahlukene ezingezansi njengokugaya ama-enzyme, i-PCR, ukwakhiwa komtapo wolwazi, i-Southern hybridization, kanye nokulandelana kwe-high-throughput.

XHUMANA NATHI

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Izici zomkhiqizo

Uhlu olubanzi lwezicelo zesampula:I-DNA ye-genomic ingakhishwa ngqo kumasampula afana negazi elingasebenzi kahle (i-EDTA, i-heparin, njll.), i-buffy coat, kanye nama-blood clots.
Kulula futhi kuyashesha:i-lysis yesampula kanye nokubopha i-nucleic acid kwenziwa ngasikhathi sinye. Ngemva kokulayisha isampula emshinini, ukukhishwa kwe-nucleic acid kuqedwa ngokuzenzakalelayo, futhi i-DNA ye-genomic esezingeni eliphezulu ingatholakala ngemizuzu engaphezu kwengu-20.
Kuphephile futhi akunabo ubuthi:I-reagent ayinazo izinyibilikisi ezinobuthi njenge-phenol ne-chloroform, futhi inesici sokuphepha esiphezulu.

Izinsimbi eziguquguqukayo

Inhlanzi Enkulu i-BFEX-32E/BFEX-32/BFEX-96E

Amapharamitha obuchwepheshe

Ubuningi besampula:200μL
Inzuzo ye-DNA:≧4μg
Ubumsulwa be-DNA:A260/280≧1.75

Imininingwane

Igama Lomkhiqizo	Ikati. Cha.	Ukupakisha
Ikhithi Yokuhlanza I-DNA Yegazi Le-MagaPure (Iphakheji Egcwalisiwe Ngaphambili)	I-BFMP02R	32T
Ikhithi Yokuhlanza I-DNA Yegazi Le-MagaPure (Iphakheji Egcwalisiwe Ngaphambili)	BFMP02R1	40T
Ikhithi Yokuhlanza I-DNA Yegazi Le-MagaPure (Iphakheji Egcwalisiwe Ngaphambili)	BFMP02R96	96T