Ikhithi Yokuhlanza I-DNA Yezilwane Ye-MagaPure

Incazelo emfushane:

Lo mkhiqizo usebenzisa uhlelo lwe-buffer oluyingqayizivele oluthuthukiswe ngokukhethekile nolulungiselelwe kahle kanye nama-magnetic beads abopha i-DNA ngqo. Ungabopha ngokushesha, umunce, uhlukanise futhi uhlanze ama-nucleic acid. Ufanele ukukhipha nokuhlanza ngempumelelo i-genomic DNA ezicutshini zezilwane ezahlukahlukene kanye nezitho zangaphakathi (kufaka phakathi izinto eziphilayo zasolwandle). Ungasusa ukungcola okufana namaprotheni ahlukahlukene, amafutha kanye nezinye izinto eziphilayo ngezinga elikhulu kakhulu. Ifakwe ithuluzi lokukhipha i-nucleic acid le-BIGFISH Magnetic Bead Method, lifanele kakhulu ukukhipha ngokuzenzakalelayo amavolumu amakhulu esampula. Imikhiqizo ye-nucleic acid ekhishwe ihlanzekile kakhulu futhi ikhwalithi enhle, futhi ingasetshenziswa kabanzi ku-PCR/qPCR engezansi, i-NGS, i-Southern hybridization kanye nolunye ucwaningo lokuhlola.

XHUMANA NATHI

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Izici zomkhiqizo

Uhlu olubanzi lwezicelo zesampula:I-DNA ye-genomic ingakhishwa ngqo kumasampula ezilwane ahlukahlukene
Kuphephile futhi akunabo ubuthi:I-reagent ayinazo izinyibilikisi ezinobuthi njenge-phenol ne-chloroform, futhi inesici sokuphepha esiphezulu.
Ukuzenzakalela:I-BIGFISH Nucleic Acid Extractor ehlonyisiwe ingenza ukukhipha okuphezulu, okufanelekile kakhulu ekukhipheni amasampula amakhulu
Ubumsulwa obuphezulu:ingasetshenziswa ngqo ku-PCR, ukugaya ama-enzyme, i-hybridization kanye nezinye izivivinyo ze-biology yamangqamuzana

Izinqubo zokukhipha

Ikhithi Yokuhlanza I-DNA Yezilwane Ehlanzekile

Izithombe zezicubu zezilwane - izithombe zokugaya nodaka - izithombe zokugeza zensimbi - izithombe zethuluzi lokukhipha i-nucleic acid
Ukuthatha isampula:Thatha izicubu zesilwane ezingama-25-30mg
Ukugaya:ukugaya i-nitrogen ewuketshezi, ukugaya noma ukusika
Ukugaya ukudla:Ukugaya okufudumele okungu-56℃
Emshinini:faka i-centrifuge bese uthatha i-supernatant, uyifake epuletini lomthombo ojulile bese uyikhipha emshinini

Amapharamitha obuchwepheshe

Isampula:25-30mg
Ubumsulwa be-DNA:A260/280≧1.75

Ithuluzi eliguquguqukayo

Inhlanzi Enkulu i-BFEX-32/BFEX-32E/BFEX-96E

Ukucaciswa komkhiqizo

Igama Lomkhiqizo	Ikati. Cha.	Ukupakisha
Ikhithi Yokuhlanza I-DNA Yezilwane Ye-MagaPure (Iphakheji Egcwalisiwe Ngaphambili)	I-BFMP01R	32T
Ikhithi Yokuhlanza I-DNA Yezicwili Zezilwane i-MagaPure (iphakheji egcwaliswe ngaphambilini)	BFMP01R1	40T
Ikhithi Yokuhlanza I-DNA Yezicwili Zezilwane i-MagaPure (iphakheji egcwaliswe ngaphambilini)	BFMP01R96	96T
I-RNase A (Ukuthenga)	I-BFRD017	1ml/pc（10mg/ml）