Ikhithi yokuhlanzwa ye-FFPE DNA

Ikhithi Yokuhlanza Ye-FFPE DNA Isithombe Esifakiwe

Incazelo emfushane:

Ikhithi isebenzisa isixazululo se-dewaxing esivumelana nemvelo ukuze kukhishwe i-DNA ye-genomic yekhwalithi ephezulu kumasampula ashumekwe upharafini, okunqoba umphumela wokuvimbela obangelwa ukuxhumanisa kwe-formalin.
Isampula ixubene ne-cell lysis buffer ukuze kuhlakazwe amaseli futhi kuvezwe i-DNA. Isampula ixubene ne-cell lysis buffer ukuphula amaseli futhi kuvezwe i-DNA. I-DNA eveziwe ithathwa yi-nanoparticles yobuhlalu obuzibuthe ukuze yakhe inkimbinkimbi ye-DNA yobuhlalu. Ubuhlalu-i-DNA complexes idluliselwa ku-The ubuhlalu-DNA complexes idluliselwa ku-wash buffer ukuze kukhishwe ukungcola okweqile. Ekugcineni, i-DNA iyahlungwa futhi itholakale ngokuyidlulisela ku-elution buffer.

XHUMANA NATHI

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Izibonelo

Izigaba ze-FFPE, amabhlogo we-FFPE, isampula kuzixazululo zokulungisa ezifana ne-formalin

Izingxenye

Imodeli	I-BFMP12R1	I-BFMP12R16	I-BFMP12R	Izingxenye
Ukupakisha	1T40T/ikhithi	16T16T/ikhithi	32T32T/ikhithi
UProtease K	400μL x 2	320μL	640μL	Isixazululo se-Protease (Kupakishwe ngokwehlukana)
RNase A	80μL	32μL	64μL	Isixazululo se-enzyme (Kupakishwe ngokwehlukana)
I-Buffer FA	12mL	5ml	10ml	Isixazululo se-saline esiphezulu
Isilondolozi FL	Kwamashumi amane nesithupha-kahle prep. i-cartridge 40 ama-pcs.	96 kuhle kupakishwe ngaphambilini ipuleti 2 ama-pcs	96 kuhle kupakishwe ngaphambilini ipuleti 2 ama-pcs.	I-denaturant eqinile nebhafa ye-Tris
Isilondolozi WA				Isixazululo se-saline esiphezulu
Isilondolozi se-WB				Isixazululo esiphansi saline
Isilondolozi DE				Isixazululo esiphansi saline
Ubuhlalu kazibuthe				Isixazululo se-Hydroxy magnetic bead
Imanuwali yabasebenzisi	1	1	1