Ikhithi Yokuhlanza I-DNA Yezilwane Zasemanzini i-MagaPure

Incazelo emfushane:

Le khithi isebenzisa uhlelo oluthile oluthuthukisiwe noluthuthukisiwe lwe-buffer kanye nama-magnetic beads abopheka ngqo kwi-DNA. Ingabopha ngokushesha, imunce, ihlukanise, futhi ihlanze ama-nucleic acid, futhi yenzelwe ngqo izilwane zasemanzini. Ifaneleka kakhulu ekukhipheni nasekuhlanzeni i-genomic DNA ngempumelelo ezicutshini zezilwane zasemanzini ezahlukahlukene, futhi ingasusa ukungcola okufana namaprotheni, amafutha, nezinye izinto eziphilayo ngezinga eliphezulu ngangokunokwenzeka. Ngokusekela ukusetshenziswa kwe-Bigfish Magnetic Bead Nucleic Acid Extractor, ifaneleka kakhulu ekukhipheni okuzenzakalelayo kosayizi abakhulu besampula. I-genomic DNA ekhishwe ihlanzekile kakhulu futhi ikhwalithi enhle, futhi ingasetshenziswa kabanzi ku-PCR/qPCR engezansi, i-NGS, i-Southern hybridization kanye nolunye ucwaningo lokuhlola.

XHUMANA NATHI

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Isingeniso esifushane

Izici zomkhiqizo

◆ Amasampula asetshenziswa kabanzi: I-DNA ye-genomic ingakhishwa ngqo kumasampula ahlukahlukene ezilwane zasemanzini
◆ Kuphephile futhi akunabo ubuthi: I-reagent ayinazo izinyibilikisi ezinobuthi ezifana ne-phenol ne-chloroform, enesici sokuphepha esiphezulu
◆ Ukuzenzakalela: Ifakwe i-Bigfish Nucleic Acid Extractor, ingenza ukukhipha okuphezulu, okufanelekile kakhulu ekukhipheni osayizi abakhulu besampula
◆ Ubumsulwa obuphezulu: bungasetshenziswa ngqo ekuhlolweni kwebhayoloji yama-molecule njenge-PCR, ukugaya ama-enzyme, ukuxutshwa, njll.

Izinqubo zokukhipha

Ukusampula: Thatha izicubu zezilwane zasemanzini ezingama-25-30 mg
Ukugaya: ukugaya i-nitrogen ewuketshezi, ukugaya noma ukucheba
Ukugaya: 56℃ ukugaya okufudumele kokugeza
Emshinini: Faka i-centrifuge ku-supernatant bese uyifaka epuletini ukuze uyikhiphe

Amapharamitha obuchwepheshe

Isampula ye-sage: 25-30mg
Ubumsulwa be-DNA: A260/280≧1.75

Ithuluzi eliguquguqukayo

Inhlanzi Enkulu i-BFEX-32/BFEX-32E/BFEX-96E

Ukucaciswa komkhiqizo

Igama Lomkhiqizo	Ikati. Cha.	Ukupakisha
I-MagaOkumsulwaI-Genomic Yezilwane ZasemanziniIkhithi Yokuhlanza I-DNA(piphakheji egcwaliswe kabusha)	I-BFMP21R	32T
I-MagaOkumsulwaI-Genomic Yezilwane ZasemanziniIkhithi Yokuhlanza I-DNA (iphakheji egcwaliswe ngaphambilini)	I-BFMP21R1	40T
I-MagaOkumsulwaI-Genomic Yezilwane ZasemanziniIkhithi Yokuhlanza I-DNA (iphakheji egcwaliswe ngaphambilini)	I-BFMP21R96	96T
I-RNase A(pi-burchase)	I-BFRD017	1ml/ipayipi (10mg/ml)