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Magacure animaatic animal genomic dna kit ukuhlanza
Isingeniso Esifushane
Le khithi imukele uhlelo oluthuthukisiwe lwe-buffer olungu-25 futhi lwenziwe kahle nobuhlalu bakamabuthe obubopha ngqo kwi-DNA. Ingabopha ngokushesha, i-adsorb, ihlukanise futhi ihlanze ama-nucleic acid, futhi yenzelwe ngqo izilwane zasemanzini. Ilungele ikakhulukazi ukukhishwa ngempumelelo kanye nokuhlanza i-genomic DNA kusuka ezicutshini zezilwane ezahlukahlukene, futhi kungasusa ukungcola okufana namaprotheni, amafutha, nezinye izinhlanganisela eziphilayo ngezinga eliphakeme okungenzeka. Ngokuxhasa ukusetshenziswa kweBigfish Magfish Bead Nucleic Acid Extractor, ilungele kakhulu ukukhishwa okuzenzakalelayo kosayizi abakhulu besampula. I-DNA ekhishwe i-genomic inobumsulwa obuphezulu nekhwalithi enhle, futhi ingasetshenziswa kabanzi ku-Downstream PCR / QPCR, ama-qp, i-perouthern hcridization nolunye ucwaningo lokuhlola.
Izici zomkhiqizo
Amasampula asetshenziswa kabanzi: I-Genomic DNA ingakhishwa ngqo kumasampula ahlukahlukene wesilwane asemanzini
◆ Kuphephile futhi okungelona ubuthi: I-realent ayinazo izixazululo ezinobuthi ezifana ne-phenol ne-chloroform, enento ephakeme yokuphepha
◆ automation: ifakwe nge-birfish nucleic acid extractor, ingangenza ukukhishwa okuphezulu, ikakhulukazi ilungele ukukhishwa osayizi abakhulu besampula
◆ Ukumsulwa okuphezulu: kungasetshenziselwa ngqo izivivinyo ze-molecular Biology ezifana ne-PCR, ukugaya kwe-enzyme, i-hybridization, njll.
Izinqubo zokukhishwa
ISampula: Thatha i-25-30 mg yezicubu zezilwane zasemanzini
Ukugaya: Ukugaya kwe-nitrogen ewuketshezi, ukugaya grinder noma ukugunda
Ukugaya: 56 ℃ ukugaywa okufudumele kokugeza
Emshinini: I-centrifuge the supernatant bese uyifaka epuletini lokukhishwa
Amapharamitha Ezobuchwepheshe
Isampula I-Sage: 25-30mg
Ukuhlanzeka kwe-DNA: A260 / 280 ≧ 1.75
Ithuluzi eliguqukayo
Bigfish bfex-32 / BFEX-32E / BFEX-96E
Ukucaciswa komkhiqizo
| Igama lomkhiqizo | Ikati. Cha. | Ukufaka ndawonye |
| Magia-NaluthoIsilwane sasemanzini genomicIkhithi yokuhlanza ye-DNA(pIphakheji egcwaliswe kabusha) | I-BFMP21R | I-32t |
| Magia-NaluthoIsilwane sasemanzini genomicIkhithi yokuhlanza ye-DNA (iphakheji egcwaliswe ngaphambili) | I-BFMP21R1 | 40T |
| Magia-NaluthoIsilwane sasemanzini genomicIkhithi yokuhlanza ye-DNA (iphakheji egcwaliswe ngaphambili) | I-BFMP21R96 | 9KOKWE |
| Rnase a(purchase) | I-Bfrd017 | I-1mL /ishubhu (I-10mg / ml) |
Izilungiselelo Zobumfihlo