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I-MagaPure Aquatic Animal Genomic DNA Purification Kit
Isingeniso esifushane
Le khithi yamukela uhlelo oluthile lwebhafa oluthuthukisiwe nolwengeziwe kanye nobuhlalu kazibuthe obubophezela ngokuqondile ku-DNA. Ingakwazi ukubopha ngokushesha, ikhangise, ihlukanise, futhi ihlanze ama-nucleic acid, futhi yakhelwe ngokukhethekile izilwane zasemanzini. Ifaneleka ngokukhethekile ekukhipheni nasekuhlanzeni i-DNA ye-genomic ezicutshini zezilwane zasemanzini ezihlukahlukene, futhi ingasusa ukungcola okunjengamaphrotheni, amafutha, nezinye izinhlanganisela eziphilayo ngokwezinga eliphezulu ngangokunokwenzeka. Ngokusekela ukusetshenziswa kwe-Bigfish Magnetic Bead Nucleic Acid Extractor, ifaneleka kakhulu ukukhishwa okuzenzakalelayo kosayizi abakhulu besampula. I-DNA ye-genomic ekhishiwe inokuhlanzeka okuphezulu kanye nekhwalithi enhle, futhi ingasetshenziswa kabanzi ku-PCR/qPCR esezansi nomfula, i-NGS, i-Southern hybridization kanye nolunye ucwaningo lokuhlola.
Izici zomkhiqizo
◆ Amasampula asetshenziswa kabanzi: I-Genomic DNA ingakhishwa ngokuqondile kumasampula ezilwane zasemanzini ahlukahlukene
◆ Iphephile futhi ayinabo ubuthi: I-reagent ayinazo izincibilikisi ezinobuthi njenge-phenol ne-chloroform, enesici sokuphepha esiphezulu.
◆ I-Automation: Ifakwe i-Bigfish Nucleic Acid Extractor, ingenza i-high-throughput extraction, ifaneleka ngokukhethekile ukukhipha amasampula amasayizi amakhulu.
◆ Ukuhlanzeka okuphezulu: kungasetshenziswa ngokuqondile ekuhlolweni kwebhayoloji yamangqamuzana njenge-PCR, ukugaya kwe-enzyme, ukuxutshwa, njll.
Izinqubo zokukhishwa
Isampula: Thatha u-25-30 mg wezicubu zezilwane zasemanzini
Ukugaya: ukugaya i-nitrogen ewuketshezi, ukugaya noma ukugunda
Ukugaya: 56℃ okugeza okufudumele kokugaya
Emshinini: Faka i-Centrifuge i-supernatant bese uyifaka epuletini ukuze uyikhiphe
Imingcele yezobuchwepheshe
Isampula do sage: 25-30mg
Ubumsulwa be-DNA: A260/280≧1.75
Insimbi eguquguqukayo
I-Bigfish BFEX-32/BFEX-32E/BFEX-96E
Ukucaciswa komkhiqizo
| Igama Lomkhiqizo | Ikati. Cha. | Ukupakisha |
| MagaOkumsulwaI-Aquatic Animal GenomicIkhithi Yokuhlanza I-DNA(piphakheji egcwaliswe kabusha) | I-BFMP21R | 32T |
| MagaOkumsulwaI-Aquatic Animal GenomicI-DNA Purification Kit (iphakheji eligcwaliswe ngaphambilini) | I-BFMP21R1 | 40T |
| MagaOkumsulwaI-Aquatic Animal GenomicI-DNA Purification Kit (iphakheji eligcwaliswe ngaphambilini) | I-BFMP21R96 | 96T |
| RNase A(pthenga) | I-BFRD017 | 1ml/ithubhu (10mg/ml) |
Izilungiselelo zobumfihlo