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Izicubu zezilwane zingahlukaniswa zibe izicubu ze-epithelial, izicubu ezixhumene, izicubu ze-muscular kanye nezicubu ze-neural ngokusho kwemvelaphi yazo, i-morphology, ukwakheka kanye nezici ezivamile zokusebenza, ezixhumene futhi zincike komunye nomunye ngezilinganiso ezahlukene ukuze zenze izitho ezihlukahlukene nezinhlelo zezilwane ukuze ziqedele imisebenzi ehlukahlukene yomzimba.
Izicubu ze-Epithelial: zakhiwe ngamaseli amaningi e-epithelial ahlelwe eduze kanye nenani elincane lamangqamuzana e-interstitial wesakhiwo esifana ne-membrane, ngokuvamile embozwe ebusweni bomzimba wesilwane kanye nomzimba wamashubhu ahlukahlukene, imigodi, amaphilisi kanye nengaphakathi langaphakathi lezinye izitho. Izicubu ze-Epithelial zinemisebenzi yokuvikela, ukugcinwa, ukukhipha kanye nokumuncwa.
Izicubu ezixhumeneyo: Zakhiwe ngamaseli kanye nenani elikhulu le-intercellular matrix. Izicubu ezixhumeneyo ezikhiqizwe yi-mesoderm wuhlobo olusatshalaliswa kakhulu futhi oluhlukahlukene lwezicubu zezilwane, okuhlanganisa izicubu ezixhumene ezixegayo, izicubu ezixhumene eziminyene, izicubu ezixhumene ne-reticular, izicubu zoqwanga, izicubu zamathambo, izicubu ze-adipose nokunye. Inemisebenzi yokusekela, ukuxhuma, ukuvikela, ukuvikela, ukulungisa kanye nokuthutha.
Izicubu zemisipha: zakhiwe amangqamuzana emisipha anamandla okunqanda. Ukuma kwamaseli emisipha kuzacile njengefayibha, ngakho-ke kubizwa nangokuthi i-muscle fiber. Umsebenzi oyinhloko we-muscle fiber ukugoqa futhi wenze ukunyakaza kwemisipha. Ngokwe-morphology kanye nesakhiwo samangqamuzana emisipha nemisebenzi ehlukene, izicubu zemisipha zingahlukaniswa zibe imisipha yamathambo (imisipha eguquguqukayo), imisipha ebushelelezi kanye nemisipha yenhliziyo.
Izicubu zezinzwa: izicubu ezakhiwe ngamaseli ezinzwa namaseli e-glial. Amangqamuzana ezinzwa amayunithi e-morphological and functional system yemizwa futhi anekhono lokuzwa ukushukuma kwangaphakathi nangaphandle futhi aqhube umfutho entweni ephilayo.
Umkhiqizo we-Bigfish
Umkhiqizo wamukela isistimu ye-buffer eyingqayizivele eyenziwe ngokukhethekile futhi eyenziwe kahle kanye nobuhlalu obuzibuthe obubopha ngokuqondile i-DNA, engabopha ngokushesha futhi ikhangise, ihlukanise futhi ihlanze ama-nucleic acid. Ilungele ukukhishwa okuphumelelayo nokuhlanzwa kwe-DNA ye-genomic kuzo zonke izinhlobo zezicubu zezilwane nezitho zangaphakathi (kuhlanganise nezinto eziphilayo zasolwandle), futhi ingasusa kakhulu zonke izinhlobo zamaprotheni, amafutha nezinye izinhlanganisela eziphilayo nokunye ukungcola. Ingasetshenziswa ne-Inhlanzi enkuluI-Magnetic Bead Method Nucleic Acid Extractor, efaneleka kakhulu ukukhishwa okuzenzakalelayo kosayizi abakhulu besampula. Imikhiqizo ye-nucleic acid ekhishiwe inokuhlanzeka okuphezulu kanye nekhwalithi, futhi ingasetshenziswa kakhulu ku-PCR/qPCR ephansi, i-NGS, i-Southern hybridisation kanye nolunye ucwaningo lokuhlola.
Izici:
Uhlu olubanzi lwamasampula: I-DNA ye-genomic ingakhishwa ngokuqondile kuzo zonke izinhlobo zamasampula ezicubu zezilwane
Iphephile futhi ayinabo ubuthi: i-reagent ayinazo izincibilikisi ezinobuthi njenge-phenol, i-chloroform, njll., enesici sokuphepha esiphezulu.
I-automation: ukufanisa ne-Bigfish Nucleic Acid Extractor kungasetshenziselwa ukukhipha okuphuma phambili, ikakhulukazi kulungele ukukhishwa kukasayizi omkhulu wesampula.
Ukuhlanzeka okuphezulu: ingasetshenziselwa izivivinyo zebhayoloji yamangqamuzana, njenge-PCR, ukugaya kwe-enzyme kanye ne-hybridisation ngqo.
Izinsimbi Ezisebenzayo: BFEX-32/BFEX-32E/BFEX-96E
Inqubo yokukhipha:
Isampula: 25-30mg wezicubu zezilwane
Ukugaya: ukugaya i-nitrogen ewuketshezi, ukugaya noma ukugunda
Ukugaya: 56℃ okugeza okufudumele kokugaya
Ukugibela: i-centrifugation ukuze ukhiphe i-supernatant, bese wengeza epuletini elijulile ukuze likhishwe ebhodini.
Idatha yokuhlola: I-30mg yamasampula ezicubu ezivela ezingxenyeni ezihlukahlukene zamagundane athathwe futhi ukukhishwa kwe-DNA nokuhlanzwa kwenziwa nge-BFMP01R ngokusho kwemiyalelo. Imiphumela yokuhlolwa ibonise ukuthi ikhithi ye-BFMP01R inenani elihle lokukhipha.
Isikhathi sokuthumela: Jul-17-2025
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