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Izicubu zezilwane zingahlukaniswa zibe izicubu ze-epithelial, izicubu ezixhumanisayo, izicubu zemisipha kanye nezicubu ze-neural ngokwemvelaphi yazo, isimo, isakhiwo kanye nezici zokusebenza ezivamile, ezixhumene futhi ezincikene ngezilinganiso ezahlukene ukuze zakhe izitho nezinhlelo ezahlukahlukene zezilwane ukuze kuqedwe imisebenzi eyahlukahlukene yomzimba.
Izicubu ze-Epithelial: zakhiwe ngamaseli amaningi e-epithelial ahlelwe eduze kanye nenani elincane lamaseli angaphakathi kwesakhiwo esifana ne-membrane, ngokuvamile ambozwe ebusweni bomzimba wesilwane kanye nomzimba wamapayipi ahlukahlukene, izimbobo, ama-capsule kanye nobuso bangaphakathi bezinye izitho. Izicubu ze-Epithelial zinemisebenzi yokuvikela, ukukhupha, ukukhipha kanye nokumunca.
Izicubu ezixhumene: Yakhiwa amaseli kanye nenani elikhulu le-matrix ephakathi kwamangqamuzana. Izicubu ezixhumene ezikhiqizwa yi-mesoderm uhlobo lwezicubu zezilwane olusakazeke kakhulu futhi oluhlukahlukene, okuhlanganisa izicubu ezixhumene ezikhululekile, izicubu ezixhumene eziqinile, izicubu ezixhumeneyo ezingemuva, izicubu ze-cartilage, izicubu zamathambo, izicubu zamafutha njalo njalo. Inemisebenzi yokusekela, ukuxhumana, ukuvikela, ukuzivikela, ukulungisa kanye nokuthutha.
Izicubu zemisipha: zakhiwe ngamaseli emisipha anekhono lokufinyela. Isimo samaseli emisipha sincane njenge-fibre, ngakho-ke sibizwa nangokuthi i-fibre yemisipha. Umsebenzi oyinhloko we-fibre yemisipha ukufinyela nokwakha ukunyakaza kwemisipha. Ngokusho kokwakheka kanye nesakhiwo samaseli emisipha kanye nemisebenzi ehlukene, izicubu zemisipha zingahlukaniswa zibe imisipha yamathambo (imisipha eguquguqukayo), imisipha ebushelelezi kanye nemisipha yenhliziyo.
Izicubu zemizwa: izicubu ezakhiwe ngamaseli ezinzwa namaseli e-glial. Amaseli ezinzwa ayizinhlayiya zesimo somzimba nezokusebenza zesimiso sezinzwa futhi anekhono lokuzwa izinto zangaphakathi nezangaphandle kanye nokwenza imizwa emzimbeni.
Umkhiqizo we-Bigfish
Lo mkhiqizo usebenzisa uhlelo oluhlukile lwe-buffer oluthuthukiswe ngokukhethekile futhi olulungiselelwe kahle kanye nama-magnetic beads abopha i-DNA ngqo, engabopha futhi imunce, ihlukanise futhi ihlanze ama-nucleic acid ngokushesha. Ifanele ukukhishwa nokuhlanzwa kwe-genomic DNA kuzo zonke izinhlobo zezicubu zezilwane nezitho zangaphakathi (kufaka phakathi izinto eziphilayo zasolwandle), futhi ingasusa ngokuphelele zonke izinhlobo zamaprotheni, amafutha kanye nezinye izinto eziphilayo kanye nokunye ukungcola. Ingasetshenziswa neInhlanzi enkuluIndlela ye-Magnetic Bead Nucleic Acid Extractor, efaneleka kakhulu ekukhishweni okuzenzakalelayo kwamasampula amakhulu. Imikhiqizo ye-nucleic acid ekhishwe ihlanzekile futhi ikhwalithi ephezulu, futhi ingasetshenziswa kabanzi ku-PCR/qPCR, NGS, Southern hybridisation kanye nolunye ucwaningo lokuhlola.
Izici:
Uhla olubanzi lwamasampula: i-DNA ye-genomic ingakhishwa ngqo kuzo zonke izinhlobo zamasampula ezicubu zezilwane
Iphephile futhi ayinabo ubuthi: i-reagent ayinazo izinyibilikisi ezinobuthi ezifana ne-phenol, i-chloroform, njll., ezinesici sokuphepha esiphezulu
Ukuzenzakalela: ukufanisa ne-Bigfish Nucleic Acid Extractor kungasetshenziswa ekukhipheni umthamo ophezulu, ofanele kakhulu ekukhipheni usayizi omkhulu wesampula
Ubumsulwa obuphezulu: ingasetshenziswa ekuhlolweni kwebhayoloji yama-molecular, njenge-PCR, ukugaya ama-enzyme kanye ne-hybridisation ngqo
Izinsimbi Ezisebenzayo: BFEX-32/BFEX-32E/BFEX-96E
Inqubo yokukhipha:
Ukusampula: 25-30mg wezicubu zezilwane
Ukugaya: ukugaya i-nitrogen ewuketshezi, ukugaya noma ukucheba
Ukugaya: 56℃ ukugaya okufudumele kokugeza
Ukufakwa ngaphakathi: ukususwa kwe-centrifugation ukuze kususwe i-supernatant, bese kufakwe epuletini elijulile ukuze kukhishwe ngaphakathi.
Idatha yokuhlola: Kuthathwe amasampula ezicubu angu-30mg avela ezingxenyeni ezahlukene zamagundane futhi kwenziwa ukukhishwa nokuhlanzwa kwe-DNA nge-BFMP01R ngokwemiyalelo. Imiphumela yokuhlola ikhombisile ukuthi ikhithi ye-BFMP01R inezinga elihle lokukhipha.
Isikhathi sokuthunyelwe: Julayi-17-2025
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