Ukukhishwa kwe-DNA yomhlabathi/indle okusheshayo nokumsulwa, okulula nge-Big Fish Sequence

Inhlabathi, njengendawo ehlukahlukene yemvelo, icebile ngezinsizakusebenza ezincane, okuhlanganisa izinhlobo eziningi zamagciwane njengebhaktheriya, isikhunta, amagciwane, i-cyanobacteria, i-actinomycetes, i-protozoa kanye ne-nematodes. Njengoba inemisebenzi eminingi ye-metabolic kanye nezakhiwo zomzimba, idlala indima ebalulekile ekujikelezeni kwezakhamzimba zomhlabathi futhi ibalulekile ekususweni kwezinto ezingcolisa umhlabathi. Njengenye yezindawo ezihluke kakhulu ngebhayoloji emhlabeni, izifundo zebhayoloji yama-molecular zenhlabathi zibaluleke kakhulu ngebhayoloji. Kule nqubo, ukuthola i-DNA yamagciwane kumasampula enhlabathi kuyisinyathelo sokuqala ocwaningweni lwenhlabathi kanye nesinyathelo esibaluleke kakhulu sempumelelo yokuhlolwa okungezansi komfula. Kodwa-ke, ngaphezu kwezinsizakusebenza ezincane ezicebile, inhlabathi ivame ukuqukethe inani elikhulu lama-metabolites (i-humic acid, i-xanthic acid nezinye izinto ze-humic), ezingahlanzwa kalula kanye nama-nucleic acid ngesikhathi senqubo yokukhipha i-nucleic acid, okuthinta inqubo ye-PCR engezansi kanye nokulandelana.Inhlanzi EnkuluI-Sequencing Soil and Fecal Genomic DNA Purification Kit ingakhipha ngempumelelo nangokushesha i-DNA ye-genomic emsulwa futhi egxile kakhulu kumasampula acebile nge-humus njengendle yomhlabathi, okuwumsizi onamandla ocwaningweni lokuhlukahluka kwezinhlobo zezinto eziphilayo zomhlabathi.

Umkhiqizo Wezinhlanzi Ezinkulu

Lo mkhiqizo usebenzisa uhlelo oluhlukile lwe-buffer oluthuthukiswe ngokukhethekile futhi olulungiselelwe kahle kanye nama-magnetic beads abopha i-DNA ngqo, engabopha futhi imunce ngokushesha, ihlukanise futhi ihlanze ama-nucleic acid, okwenza kube kuhle kakhulu ekuhlukaniseni nasekuhlanzeni i-genomic DNA ngokushesha nangendlela ephumelelayo enhlabathini nasendle, kanye nokususwa kwezinsalela ezifana nama-humic acid, amaprotheni, ama-ion kasawoti, kanye nezinye izinsalela. Uma kuqhathaniswa ne-Beaglefly sequencing magnetic bead method nucleic acid extractor, ifaneleka kakhulu ekukhishweni okuzenzakalelayo kosayizi omkhulu wesampula. I-genomic DNA ekhishwe ihlanzekile kakhulu futhi ikhwalithi ephezulu futhi ingasetshenziswa kabanzi ku-PCR/qPCR, NGS nakwezinye izicwaningo zokuhlola.

Izici

Ikhwalithi enhle:ukuhlukaniswa nokuhlanzwa kwe-DNA ye-genomic, isivuno esikhulu, ubumsulwa obuhle.

Uhla olubanzi lwamasampula:ingasetshenziswa kabanzi kuzo zonke izinhlobo zenhlabathi kanye namasampula endle.

Kulula futhi kuyashesha:ukukhishwa okuzenzakalelayo nge-extractor efanayo, efanelekile kakhulu ekukhishweni kwamasampula amakhulu.

Kuphephile futhi akunabo ubuthi:asikho isidingo sama-reagent anobuthi e-organic njenge-phenol/chloroform, njll.

Izinsimbi eziguquguqukayo:I-BFEX-32/ BFEX-32E/ BFEX-96E


Isikhathi sokuthunyelwe: Julayi-10-2025
Izilungiselelo zobumfihlo
Phatha Imvume Yamakhukhi
Ukuze sinikeze okuhlangenwe nakho okuhle kakhulu, sisebenzisa ubuchwepheshe obufana namakhukhi ukugcina kanye/noma ukufinyelela ulwazi lwedivayisi. Ukuvuma lobu buchwepheshe kuzosivumela ukuthi sicubungule idatha efana nokuziphatha kokuphequlula noma ama-ID ahlukile kulesi siza. Ukungavumi noma ukuhoxisa imvume, kungathinta kabi izici nemisebenzi ethile.
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