I-Ultramicrospectrophotometer

Isingeniso Somkhiqizo

I-Ultramicrospectrophotometer iwuhlobo lokutholwa okusheshayo nokunembile kwe-nucleic acid, amaprotheni kanye nokugxilwa kwesisombululo seseli ngaphandle kokushisisa, usayizi wesampula kuphela 0.5 kuya ku-2ul, futhi imodi ye-cuvette ingathola ukugxila kwamabhaktheriya neminye imidiya yesiko. Umsebenzi wokuthola i-Fluorescence ungamataniswa nekhithi yokuhlaziya ubuningi be-fluorescence, ngenhlanganisela ethile yodayi be-fluorescent nezinto eziqondiwe zingakala ngokunembile ubuningi be-DNA, i-RNA namaphrotheni, futhi ubuncane bungafinyelela ku-0.5pg/μl (dsDNA).

Izici zomkhiqizo

Imvamisa yokucwayiza komthombo wokukhanya mfishane, uma kuqhathaniswa nendlela yokutholwa evamile yokwandisa impilo yesevisi yomthombo wokukhanya. Ukukhuthazwa kokukhanya kwemikhiqizo emincane yokuhlola kungase kutholakale ngokushesha, akulula ukwehliswa;

Umsebenzi we-fluorescence: Nge-fluorescence quantitative reagent ingathola i-pg concentration ye-dsDNA;

Ubuchwepheshe bokuthola indlela ye-4 optical: ubuchwepheshe obuhlukile bokulawula imoto, ukusetshenziswa kwemodi yokubona indlela "4", ukuzinza, ukuphindaphinda, umugqa ungcono, ububanzi bokulinganisa bukhulu;

Iphrinta eyakhelwe ngaphakathi: Ngezinketho ezisebenziseka kalula zokusebenzisa idatha kuya kwiphrinta, ungaphrinta imibiko ngokuqondile emshinini wokuphrinta owakhelwe ngaphakathi.r;

Isixazululo sebhaktheriya se-OD600, ukutholwa kwe-microbial: ngohlelo lokutholwa kwendlela ye-OD600, kunemodi yesitsha ilungele amagciwane, ama-microorganisms nokunye ukutholwa kwesixazululo samasiko.;

Ukuphindaphinda okuphezulu kanye nomugqa;