I-Ultramicrospectrophotometer

Isingeniso Somkhiqizo

I-Ultramicrospectrophotometer uhlobo lokuthola okusheshayo nokunembile kwe-nucleic acid, amaprotheni kanye nokuhlushwa kwesisombululo seseli ngaphandle kokushisa kwangaphambili, usayizi wesampula u-0.5 kuya ku-2ul kuphela, futhi imodi ye-cuvette ingathola ukuhlushwa kwamagciwane kanye nezinye izinto zokukhulisa. Umsebenzi wokuthola i-fluorescence ungahlanganiswa nekhithi yokuhlaziya inani le-fluorescence, ngokuhlanganiswa okuqondile kwamadayi e-fluorescent nezinto eziqondiwe kungalinganisa ngokunembile ukuhlushwa kwe-DNA, i-RNA kanye namaprotheni, futhi ubuncane bungafinyelela ku-0.5pg/μl (dsDNA).

Izici zomkhiqizo

Imvamisa yokufiphaza komthombo wokukhanya imfushane, uma iqhathaniswa nendlela yendabuko yokuthola ukwandisa impilo yesevisi yomthombo wokukhanya. Ukukhuthazwa kokukhanya kwemikhiqizo emincane yokuhlola kungaba ukutholwa okusheshayo, akulula ukukuhlanekezela.;

Umsebenzi we-Fluorescence: Nge-fluorescence, i-reagent yokulinganisa ingathola ukuhlushwa kwe-pg dsDNA;

Ubuchwepheshe bokuthola indlela yokukhanya obune: ubuchwepheshe bokulawula izimoto obuhlukile, ukusetshenziswa kwemodi yokuthola indlela yokukhanya "4", ukuzinza, ukuphindaphindeka, ukulingana kungcono, ububanzi bokulinganisa bukhulu;

Iphrinta eyakhelwe ngaphakathi: Ngezinketho zedatha ezisebenziseka kalula, ungaphrinta imibiko ngqo kusuka kuphrinta eyakhelwe ngaphakathir;

Isixazululo samabhaktheriya se-OD600, ukutholwa kwamagciwane: ngohlelo lokuthola indlela ye-OD600, kunemodi yesitsha elungele amagciwane, amagciwane kanye nokunye ukutholwa kokugxila kwesisombululo samasiko;

Ukuphindaphinda okuphezulu kanye nokuhleleka;