I-MagaPure Animal Tissue Genomic DNA Purification Kit

Incazelo emfushane:

Lo mkhiqizo usebenzisa isistimu yebhafa ethuthukiswe ngokukhethekile futhi elungiselelwe kanye nobuhlalu obuzibuthe obuhlanganisa ngokuqondile i-DNA. Ingakwazi ukubopha ngokushesha, i-adsorb, ihlukanise futhi ihlanze ama-nucleic acid. Ilungele ukukhipha nokuhlanza i-DNA ye-genomic ezinhlobonhlobo zezicubu zezilwane nezitho zangaphakathi (kuhlanganise nezinto eziphilayo zasolwandle). Ingasusa ukungcola okufana namaprotheni ahlukahlukene, amafutha nezinye izinhlanganisela eziphilayo ngokwezinga elikhulu. Ifakwe ithuluzi le-BIGFISH Magnetic Bead Method nucleic acid extraction, ifaneleka kakhulu ukukhishwa okuzenzakalelayo kwamavolumu amakhulu amasampula. Imikhiqizo ye-nucleic acid ekhishiwe inokuhlanzeka okuphezulu kanye nekhwalithi enhle, futhi ingasetshenziswa kabanzi ku-PCR/qPCR ephansi, i-NGS, i-Southern hybridization kanye nolunye ucwaningo lokuhlola.

XHUMANA NATHI

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Izici zomkhiqizo

Uhlu olubanzi lwezinhlelo zokusebenza zesampula:I-Genomic DNA ingakhishwa ngokuqondile kumasampula ezilwane ahlukahlukene
Iphephile futhi ayinabo ubuthi:I-reagent ayinazo izincibilikisi ezinobuthi njenge-phenol ne-chloroform, futhi inesici sokuphepha esiphezulu.
Okuzenzakalelayo:I-BIGFISH Nucleic Acid Extractor ehlomile ingenza i-high-throughput extract, ifanelekele ukukhishwa kwesampula enkulu.
Ukuhlanzeka okuphezulu:ingasetshenziswa ngokuqondile ku-PCR, ukugaya kwe-enzyme, ukuxutshwa nokunye ukuhlola kwebhayoloji yamangqamuzana

Izinqubo zokukhishwa

I-MagaPure-Animal-Tissue-Genomic-DNA-Purification-Kit

Izithombe zezicubu zezilwane - izithombe zokugaya nodaka - izithombe zokugeza insimbi - izithombe zensimbi yokukhipha i-nucleic acid
Ukusampula:Thatha izicubu zezilwane ezingama-25-30mg
Ukugaya:ukugaya i-nitrogen ewuketshezi, ukugaya i-grinder noma ukusika
Ukugaya:56℃ ukugaya okugeza okufudumele
Emshinini:centrifuge bese uthatha i-supernatant, uyifake epuleti lomthombo ojulile bese uyikhipha emshinini

Imingcele yezobuchwepheshe

Isampula:25-30mg
Ubumsulwa be-DNA:I-A260/280≧1.75

Insimbi eguquguqukayo

I-Bigfish BFEX-32/BFEX-32E/BFEX-96E

Ukucaciswa komkhiqizo

Igama Lomkhiqizo	Ikati.No.	Ukupakisha
I-MagaPure Animal Tissue Genomic DNA Purification Kit (iphakheji eligcwaliswe ngaphambilini)	I-BFMP01R	32T
I-MagaPure Animal Tissue Genomic DNA Purification Kit (iphakheji eligcwaliswe ngaphambilini)	I-BFMP01R1	40T
I-MagaPure Animal Tissue Genomic DNA Purification Kit (iphakheji eligcwaliswe ngaphambilini)	I-BFMP01R96	96T
I-RNase A (Thenga)	I-BFRD017	1ml/pc (10mg/ml)