I-Magpire bacteria genomic DNA yokuhlanza ikhithi yokuhlanza

I-Magpire bacteria genomic DNA yokuhlanza ikhithi efakwe isithombe

Incazelo emfushane:

Ngemuva kokuphathwa ngoketshezi lokugaya ukudla, i-nucleic acid ekhasini le-bacterium likhishwe kuphela kusetshenziswa i-lysis buffer. I-genomic DNA iboshelwe kuphela futhi ikakhulukazi kuma-Magnetic Beads. I-genomic DNA eboshelwe izinhlayiyana kazibuthe iyabanjwa yizinto ezibucayi; Ukungcola kususwa ngokugeza nge-wash buffer. I-acid ye-nucleic isenyuswa yizinhlayiya ezinesiqeshana. Kusebenza kuzo zonke izinhlobo zamagciwane angenamagagasi ama-bacterium alungile, njll.

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Isakhi esiyinhloko

Ikati.#	I-BFMP10R1	I-BFMP10R16	I-BFMP10R	COkuhle
Paokokuqala	1T40T / Kit	16T16T / Kit	32T32t / kit
Proteise k	I-400μ l x 2	320μ l	640μ l	Isixazululo se-Protease (sipakishwe ngokwehlukile)
I-RNASEA	I-80μ l	32μ l	64μ l	Isixazululo se-enzyme (sigcwele ngokwehlukile)
Buffer ba	I-12ml	I-5ML	I-10ml	Isixazululo se-Saline
Buffer bl	Kumashumi amane 6-kahle prep. igobolondo Ama-40 ama-PC.	96 Well Kugcwele phama ipuleti Ama-2 ama-PC.	96 Well Kugcwele phama ipuleti Ama-2 ama-PC.	I-stroturant eqinile ne-tris buffer
Buffer wa				Isixazululo se-Saline
Buffer wb				Isixazululo se-saline esiphansi
Buffer de				Isixazululo se-Tris
Ama-Magneticbeads				Isixazululo se-Hydroxy Magnetic Bead Solution
Imali ebhukwayo	1	1	1